Related Experiment Video
Updated: Feb 18, 2026
Immunostimulatory Agent Evaluation: Lymphoid Tissue Extraction and Injection Route-Dependent Dendritic Cell Activation
Published on: September 16, 2018
Direct intranasal application of the solid phase of ImmunoCAP® increases nasal specific immunoglobulin E detection in
Paloma Campo1, María Del Carmen Plaza-Seron2, Ibon Eguiluz-Gracia1
1Allergy Unit, Instituto de Investigación Biomédica de Málaga (IBIMA)-Regional University Hospital of Málaga, Malaga, Spain.
Background:
The measurement of nasal specific IgE (NsIgE) in local allergic rhinitis (LAR) patients is challenging and shows variability. The objective of this work was to evaluate a minimally-invasive method of direct detection of NsIgE in patients with LAR to Dermatophagoides pteronyssinus (DP) using an automated immunoassay.
Methods:
Fifty patients participated (LAR, n = 14; allergic rhinitis (AR), n = 20; healthy controls [HC], n = 16). Detection of NsIgE was performed by direct application of the solid phase of a commercial DP ImmunoCAP® test 24 hours after DP nasal provocation.
Results:
There was no difference in the median volume of secretion absorbed by the solid phase of the ImmunoCAP test in the 3 studied groups (p = 0.17). According to receiver operating characteristic (ROC) curve analysis, NsIgE ≥0.1450 was the optimal cutoff point, obtaining in LAR patients 42.86% sensitivity with the highest specificity (100%), and 75% sensitivity and 100% specificity for AR.
Conclusion:
This study demonstrates the detection of NsIgE to DP in LAR by using a simple, commercial device with high specificity.
