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Author Spotlight: Advancing Mitochondrial Research - mtHyper7 Biosensor for Subcellular Analysis
Published on: June 2, 2023
A mitochondria-targeted ratiometric two-photon fluorescent probe for detecting intracellular cysteine and
Ping Yue1, Xiuli Yang2, Peng Ning1
1School of Chemistry and Chemical Engineering & Center for Atomic Engineering of Advanced Materials & AnHui Province Key Laboratory of Chemistry for Inorganic/Organic Hybrid Functionalized Materials, Anhui University, Hefei 230601, PR China.
Abstract:
A novel mitochondria-targeted ratiometric two-photon fluorescent probe (Mito-MQ) for detecting intracellular cysteine (Cys) and homocysteine (Hcy) has been designed. Mito-MQ showed the ratiometric fluorescent detection signal (the green-to-blue emissionfrom 517nm to 460nm) to cysteine (Cys) and homocysteine (Hcy) over glutathione (GSH), along with the fast response rate (10min). The detection mechanism was illustrated by 1H NMR, ESI-MS and theoretical calculation. The co-localization coefficient of 0.87 between Mito-MQ and MitoTracker Red revealed that the probe was predominantly present in mitochondria, therefore, Mito-MQ was successfully applied to detect mitochondrial oxidative stress by detecting the change of Cys/Hcy. Moreover, imaging in fresh tissue slices indicated that Mito-MQ could work in deep tissue (ca. 130µm) under two-photon excitation. Furthermore, the measurement of Cys/Hcy detection in zebrafish showed that probe can be used in determination of biothiols in vivo.
