Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR/Cas9 Genome Editing01:28

CRISPR/Cas9 Genome Editing

2.0K
The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
2.0K
CRISPR01:59

CRISPR

58.1K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.1K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.3K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.3K
Homologous Recombination02:31

Homologous Recombination

63.9K
The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...
63.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Potential rules for stable transition metal hexafluorides with high oxidation states under high pressures.

Physical chemistry chemical physics : PCCP·2023
Same author

Stable multifunctional aluminum phosphides at high pressures.

Physical chemistry chemical physics : PCCP·2023
Same author

A novel visceral adiposity index predicts bone loss in female early rheumatoid arthritis patients detected by HR-pQCT.

Scientific reports·2023
Same author

A copper-metal organic framework enhances the photothermal and chemodynamic properties of polydopamine for melanoma therapy.

Acta biomaterialia·2023
Same author

Triplex-forming oligonucleotides as an anti-gene technique for cancer therapy.

Frontiers in pharmacology·2023
Same author

A SiO<sub>2</sub> layer on PEO-treated Mg for enhanced corrosion resistance and bone regeneration.

Frontiers in bioengineering and biotechnology·2023

Related Experiment Video

Updated: Feb 18, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.9K

Efficient gene editing in Corynebacterium glutamicum using the CRISPR/Cas9 system.

Feng Peng1,2,3, Xinyue Wang1,2,3, Yang Sun1,2,3

  • 1National Engineering Laboratory for Cereal Fermentation Technology, Jiangnan University, Wuxi, 214122, China.

Microbial Cell Factories
|November 16, 2017
PubMed
Summary

We developed a CRISPR/Cas9 system for efficient genome editing in Corynebacterium glutamicum. This tool enables rapid gene deletion and insertion, improving recombinant protein expression and facilitating genetic studies.

Keywords:
CRISPR/Cas9Corynebacterium glutamicumGenome editingProtein expression

More Related Videos

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.5K
CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
07:25

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

10.2K

Related Experiment Videos

Last Updated: Feb 18, 2026

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
09:51

Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms

Published on: May 25, 2018

35.9K
CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.
07:46

CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

Published on: December 11, 2020

6.5K
CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis
07:25

CRISPR-Cas9-Mediated Genome Editing in the Filamentous Ascomycete Huntiella omanensis

Published on: June 9, 2020

10.2K

Area of Science:

  • Microbiology
  • Molecular Biology
  • Biotechnology

Background:

  • Corynebacterium glutamicum is a key microbial host for industrial production.
  • Genetic modification is crucial for improving C. glutamicum's capabilities.
  • Gene editing techniques are essential for chromosomal modification.

Purpose of the Study:

  • To establish a CRISPR/Cas9 system for genome editing in C. glutamicum.
  • To enable rapid and efficient gene deletion and insertion.
  • To enhance recombinant protein expression in C. glutamicum.

Main Methods:

  • Developed a two-plasmid CRISPR/Cas9 system.
  • Utilized a target-specific guide RNA and homologous sequence.
  • Expressed Cas9 protein for genome editing.

Main Results:

  • Achieved high-efficiency (up to 100%) gene editing.
  • Successfully disrupted porB, mepA, clpX, and Ncgl0911 genes.
  • Demonstrated enhanced green fluorescent protein expression in deletion strains.

Conclusions:

  • Adapted the CRISPR/Cas9 system for C. glutamicum.
  • Provided a rapid and efficient genome editing tool.
  • Facilitated gene function studies, metabolic pathway analysis, and productivity enhancement.