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Evaluation of a pointwise microcirculation assessment method using liquid and multilayered tissue simulating

Ingemar Fredriksson1,2, Rolf B Saager3, Anthony J Durkin3,4

  • 1Linköping University, Department of Biomedical Engineering, Linköping, Sweden.

Journal of Biomedical Optics
|November 16, 2017
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Summary

This study validates a fiber-optic probe for measuring skin microcirculation. The instrument accurately quantifies red blood cell fraction and oxygen saturation in tissue phantoms, crucial for assessing blood flow.

Keywords:
diffuse reflectance spectroscopyinverse Monte Carlomicrocirculationmultilayered tissue modeloptical phantomssampling volume

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Area of Science:

  • Biomedical Optics
  • Medical Instrumentation
  • Microcirculation Research

Background:

  • Assessing microcirculation parameters like red blood cell tissue fraction (fRBC) and oxygen saturation (SO2) is vital for understanding tissue health.
  • Existing methods may have limitations in accuracy and real-time measurement.
  • A novel fiber-optic probe integrating diffuse reflectance spectroscopy (DRS) and laser Doppler flowmetry offers potential for improved assessment.

Purpose of the Study:

  • To evaluate the accuracy of a fiber-optic probe-based instrument for measuring key microcirculation parameters.
  • To characterize the performance of the diffuse reflectance spectroscopy (DRS) component using advanced tissue phantoms.
  • To validate the instrument's ability to estimate red blood cell tissue fraction (fRBC), oxygen saturation (SO2), reduced scattering coefficient (μs'), and average vessel diameter (D).

Main Methods:

  • Utilized a fiber-optic probe integrating DRS (two source-detector separations) and laser Doppler flowmetry.
  • Employed an inverse Monte Carlo method for parameter identification within a multilayered tissue model.
  • Validated the DRS accuracy using liquid blood phantoms and solid epidermis-dermis mimicking phantoms (polydimethylsiloxane, titanium oxide, hemoglobin, coffee).

Main Results:

  • Root-mean-square (RMS) deviations for fRBC were 11% and 5.3% (liquid phantoms), and 11% (solid phantoms).
  • RMS deviations for SO2 were 5.2% and 2.9% (liquid phantoms), and 2.9% (solid phantoms).
  • RMS deviation for μs' was 15% (solid phantoms, 475-850 nm), and for average vessel diameter (D) was 1 μm (liquid phantoms).

Conclusions:

  • The fiber-optic probe demonstrates reasonable accuracy in estimating skin microcirculation parameters.
  • Validated parameters include red blood cell tissue fraction (fRBC) and oxygen saturation (SO2).
  • The instrument also accurately estimates optical properties (μs') and average vessel diameter (D) in phantoms, indicating its potential for clinical application.