Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Fluorescence energy transfer detects changes in fibronectin structure upon surface binding.

C Wolff1, C S Lai

  • 1Department of Radiology, Medical College of Wisconsin, Milwaukee 53226.

Archives of Biochemistry and Biophysics
|February 1, 1989
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Streptococcus dysgalactiae ssp. dysgalactiae in Norwegian bovine dairy herds: Risk factors, sources, and genomic diversity.

Journal of dairy science·2022
Same author

Uncertainty and Bias in Global to Regional Scale Assessments of Current and Future Coastal Flood Risk.

Earth's future·2021
Same author

Stimulated plasmon polariton scattering.

Nature communications·2020
Same author

World Trade Organization agreements: a framework for trade in animals and animal products.

Revue scientifique et technique (International Office of Epizootics)·2020
Same author

Introduction : Ensuring safe trade in animals and animal products.

Revue scientifique et technique (International Office of Epizootics)·2020
Same author

Role of diffusive surface scattering in nonlocal plasmonics.

Journal of physics. Condensed matter : an Institute of Physics journal·2020
Same journal

Protocatechuic acid promotes c-Myc-mediated cell proliferation by directly targeting TRAF2.

Archives of biochemistry and biophysics·2026
Same journal

Targeting CD8<sup>+</sup> T cell-derived granzyme K alleviates radiation-induced pulmonary fibrosis by attenuating senescence and SASP.

Archives of biochemistry and biophysics·2026
Same journal

The Binding Affinity and Recognition Specificity of Dopamine to Human Dopamine Receptors can be Rationally Altered by Halogen Doping.

Archives of biochemistry and biophysics·2026
Same journal

Cortical Cholesterol Deficit and Reduced Network Excitability in ApoE-Deficient Mice.

Archives of biochemistry and biophysics·2026
Same journal

Unraveling zinc's impact: Variable effects across concentrations in Phaseolus coccineus thylakoids.

Archives of biochemistry and biophysics·2026
Same journal

Characterizing tumor-associated endothelial cells in clear cell renal cell carcinoma via scRNA-seq and Bulk RNA-seq: construction of a prognostic risk signature.

Archives of biochemistry and biophysics·2026
See all related articles

Human plasma fibronectin (Fn) undergoes significant conformational changes upon surface binding. Adsorption to microcarriers dramatically increases intramolecular distances, suggesting a transition from a compact to an extended form, potentially important for Fn activation.

Area of Science:

  • Biochemistry
  • Protein conformational analysis
  • Surface science

Background:

  • Human plasma fibronectin (Fn) is a crucial extracellular matrix protein.
  • Understanding Fn's conformational states is key to its biological functions.
  • Protein adsorption to surfaces can alter molecular structure and activity.

Purpose of the Study:

  • To investigate intramolecular distance changes in human plasma fibronectin upon surface adsorption.
  • To determine if surface binding induces conformational alterations in Fn.
  • To explore the implications of these changes for fibronectin activation.

Main Methods:

  • Utilized fluorescence energy transfer (FRET) techniques to measure intramolecular distances.
  • Enzymatically labeled specific residues (glutamine-3, free sulfhydryl groups) with donor/acceptor fluorophores.

Related Experiment Videos

  • Employed Cytodex dextran microcarriers for protein adsorption studies.
  • Performed steady-state fluorescence measurements in solution and upon surface binding.

    • Main Results:

    • In solution, Fn's amino termini were juxtaposed (23 Å).
    • Upon adsorption to microcarriers, the distance between amino termini increased to over 70 Å, indicated by abolished FRET.
    • Labeling of amino termini and sulfhydryl groups showed distances within 70 Å in solution, decreasing upon surface binding.
    • Surface binding induced a significant conformational change, likely from compact to extended.

    Conclusions:

    • Plasma fibronectin undergoes a drastic conformational change upon adsorption to surfaces.
    • This transition involves a significant increase in intramolecular distances, suggesting unfolding or extension.
    • The conformational change upon surface binding may be critical for fibronectin's biological activity and activation.