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Comparative genomics of type 1 IncC plasmids from China
Lizhi Ma1, Zhe Yin2, Defu Zhang2,3
1Department of Emergency Medicine, General Hospital of Chinese People's Armed Police Forces, Beijing 100039, China.
Future Microbiology
|November 16, 2017
Summary
Genomic characterization of Chinese IncC resistance plasmids reveals distinct lineages. Complex rearrangements in p112298-tetA differentiate it from other IncC plasmids, highlighting plasmid evolution.
Area of Science:
- Genomics
- Molecular Biology
- Microbiology
Background:
- IncC plasmids are known for their broad host range and ability to disseminate antibiotic resistance genes across different bacterial species.
- Understanding the genomic diversity and evolution of these plasmids is crucial for combating antimicrobial resistance.
Purpose of the Study:
- To perform a detailed genomic characterization of a type 1 IncC resistance plasmid from China.
- To compare its genetic structure with other IncC plasmids to understand their evolutionary relationships and the dissemination of resistance genes.
Main Methods:
- Whole-genome sequencing of the plasmid p112298-tetA.
- Comparative genomic analysis with reference IncC plasmids (pR148) and other sequenced IncC plasmids from China (pHS36-NDM, pVAS3-1).
Main Results:
- The studied plasmid, p112298-tetA, contains multiple exogenous resistance islands, including ARI-A, ARI-B, ISEcp1-blaCMY, and blaKPC-2.
- These resistance islands are integrated at various positions within the IncC backbone, defining three distinct plasmid lineages.
- Significant genomic rearrangements and homologous recombination events were observed in p112298-tetA, leading to substantial modular differences compared to the other analyzed plasmids.
Conclusions:
- The evolution of IncC plasmids involves complex rearrangement and recombination processes.
- These events contribute to the modular diversity of both the plasmid backbone and the acquired resistance regions.
- The findings provide insights into the mechanisms driving the spread and evolution of antibiotic resistance mediated by IncC plasmids.
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