Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.7K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.7K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.2K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.2K
Introduction to Nuclear Reprogramming01:14

Introduction to Nuclear Reprogramming

2.3K
Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
2.3K
Cytotoxic T Cells-mediated Immune Response01:27

Cytotoxic T Cells-mediated Immune Response

7.6K
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
7.6K
T Cell Activation and Clonal Selection01:22

T Cell Activation and Clonal Selection

16.4K
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
16.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sequential infection reprograms the immune landscape to shape future responses.

bioRxiv : the preprint server for biology·2026
Same author

Type I interferons enhance B cell activation and promote differentiation of double negative 2 cells in SLE.

Lupus science & medicine·2026
Same author

Functional Type I and Type II interferon crosstalk restricts progenitor exhausted CD8 T cells through spatial exclusion and checkpoint enforcement.

bioRxiv : the preprint server for biology·2026
Same author

Lentivirus-based HPSC therapy provides effective and long-term treatment in hypophosphatasia mouse model.

Molecular therapy : the journal of the American Society of Gene Therapy·2026
Same author

Stepwise differentiation from precursor intermediates and distinct Th1 checkpoints promote CD4 Th1 cell differentiation during chronic viral infection.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

A proimmunotoxin nanodrug targeting AIDS-associated non-Hodgkin lymphoma.

Journal of controlled release : official journal of the Controlled Release Society·2026

Related Experiment Video

Updated: Feb 18, 2026

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice
07:25

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice

Published on: September 25, 2019

7.4K

Purging Exhausted Virus-Specific CD8 T Cell Phenotypes by Somatic Cell Reprogramming.

Joshua Chan1, Patrick Y Kim2, Emiko Kranz2

  • 11 Division of Hematology and Oncology, David Geffen School of Medicine at UCLA , Los Angeles, California.

AIDS Research and Human Retroviruses
|November 16, 2017
PubMed
Summary
This summary is machine-generated.

Scientists rejuvenated exhausted T cells, critical for fighting viruses, into induced pluripotent stem cells (iPSCs). These iPSCs regenerated functional, virus-specific T cells, offering a new approach for chronic infections.

Keywords:
CD8 T cellLCMVT cell exhaustionchronic infectioniPSCsomatic cell reprogramming

More Related Videos

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes
10:03

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

Published on: November 11, 2016

10.3K
Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype
08:49

Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype

Published on: March 18, 2020

11.7K

Related Experiment Videos

Last Updated: Feb 18, 2026

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice
07:25

Stem Cell-Derived Viral Ag-Specific T Lymphocytes Suppress HBV Replication in Mice

Published on: September 25, 2019

7.4K
Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes
10:03

Generation of Induced Pluripotent Stem Cells from Human Melanoma Tumor-infiltrating Lymphocytes

Published on: November 11, 2016

10.3K
Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype
08:49

Transduction and Expansion of Primary T Cells in Nine Days with Maintenance of Central Memory Phenotype

Published on: March 18, 2020

11.7K

Area of Science:

  • Immunology
  • Stem Cell Biology
  • Virology

Background:

  • Cytotoxic T cells are vital for controlling viral infections.
  • Chronic viral infections lead to T cell exhaustion, impairing immune response and causing viral persistence.
  • Existing treatments for chronic viral infections remain limited.

Purpose of the Study:

  • To investigate if somatic cell reprogramming can rejuvenate exhausted T cells.
  • To regenerate functional T cells from exhausted T cells for potential therapeutic applications.
  • To establish a proof-of-concept for reversing T cell exhaustion.

Main Methods:

  • Generation of a novel mouse line (B6/129OKSM) with inducible reprogramming genes.
  • Induction of chronic lymphocytic choriomeningitis virus (LCMV) infection.
  • Ex vivo reprogramming of exhausted LCMV-specific T cells into induced pluripotent stem cells (iPSCs) using doxycycline.
  • Blastocyst injection of reprogrammed iPSCs and subsequent transfer to foster mothers.
  • Analysis of differentiated T cells for functionality and antigen specificity.

Main Results:

  • Exhausted T cells from chronically infected mice were successfully reprogrammed into iPSCs.
  • Reprogrammed iPSCs differentiated into functional naive T cells.
  • The regenerated T cells retained their original antigen specificity.
  • The reprogramming process erased the imprints of the exhausted T cell state.

Conclusions:

  • Somatic cell reprogramming offers a viable strategy to reverse T cell exhaustion.
  • This approach can regenerate functional, virus-specific T cells from exhausted precursors.
  • This study provides a novel therapeutic avenue for managing chronic viral infections.