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Related Experiment Video

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Multiplexed Fluorescent Microarray for Human Salivary Protein Analysis Using Polymer Microspheres and Fiber-optic Bundles
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Fiber-optic array using molecularly imprinted microspheres for antibiotic analysis.

Sergio Carrasco1, Elena Benito-Peña1,2, David R Walt2

  • 1Department of Analytical Chemistry , Faculty of Chemistry , Complutense University , Ciudad Universitaria s/n , Madrid 28040 , Spain . Email: elenabp@ucm.es ; Email: mcmbondi@ucm.es ; ; Tel: +34-91394-5147.

Chemical Science
|November 17, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces novel molecularly imprinted polymer (MIP) microsphere sensors for detecting the antibiotic enrofloxacin (ENRO). These biomimetic sensors enable high-density optical microarrays for sensitive and selective enrofloxacin detection.

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Area of Science:

  • Biomimetic Sensors
  • Analytical Chemistry
  • Materials Science

Background:

  • Enrofloxacin (ENRO) is an antibiotic with widespread use in human and veterinary medicine.
  • Accurate detection of ENRO is crucial for monitoring its levels and ensuring safety.
  • Existing detection methods may lack the sensitivity, selectivity, or multiplexing capabilities required for comprehensive analysis.

Purpose of the Study:

  • To develop a novel high-density optical microarray platform for sensitive and selective enrofloxacin detection.
  • To utilize molecularly imprinted polymer (MIP) microspheres as a biomimetic recognition element.
  • To demonstrate the feasibility of multiplexed detection using fluorescently encoded microspheres.

Main Methods:

  • Preparation of molecularly imprinted (MIP) and non-imprinted (NIP) polymer microspheres via thermal precipitation-polymerization.
  • Fluorescent encoding of microspheres using coumarin-30 or [Ru(dip)3]Cl2 for distinguishability.
  • Integration of MIP/NIP microspheres into an optical fiber bundle with microwells for imaging.
  • Development of a competitive immunoassay format using fluorescently labeled ENRO.

Main Results:

  • The developed MIP-based optical microarray achieved a limit of detection (LOD) of 0.04 μM for ENRO.
  • A dynamic range of 0.29-21.54 μM was established, demonstrating quantitative detection capabilities.
  • The assay showed good selectivity against other fluoroquinolones and non-related antibiotics.

Conclusions:

  • Molecularly imprinted polymers (MIPs) can serve as effective biomimetic receptors in sensor development, replacing traditional biomolecules.
  • The developed fiber-optic microarray platform enables multiplexed and sensitive detection of enrofloxacin.
  • This work represents a significant advancement towards a new generation of biomimetic sensors for antibiotic monitoring.