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Related Experiment Videos

Optimized microturbidimetric assay for fibrinogen.

M Macart1, A Koffi, G Henocque

  • 1Laboratoire du Centre Hospitalier de Laon, France.

Clinical Chemistry
|February 1, 1989
PubMed
Summary

This study presents a new assay for measuring plasma fibrinogen levels using a precipitation method. The assay is accurate, precise, and minimally affected by common interfering substances, offering reliable results for clinical diagnostics.

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Area of Science:

  • Clinical Chemistry
  • Biochemistry
  • Hematology

Background:

  • Accurate quantification of plasma fibrinogen is crucial for diagnosing and managing various hemostatic disorders.
  • Existing methods for fibrinogen measurement can be susceptible to interference from various substances.
  • Development of a robust and reliable fibrinogen assay is needed.

Purpose of the Study:

  • To develop and validate a novel turbidimetric assay for plasma fibrinogen quantification.
  • To assess the interference of common substances and monoclonal proteins on the assay's performance.
  • To evaluate the assay's precision and correlation with established methods.

Main Methods:

  • Plasma fibrinogen was precipitated using a reagent containing ammonium sulfate, EDTA, and guanidine hydrochloride.

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  • A two-step reagent addition and fixed reaction times were employed to minimize interference.
  • The assay was calibrated with purified fibrinogen and validated against immunoturbidimetric, chronometric, and clotting assays.

    • Main Results:

    • The assay demonstrated linear response from 0-10 g/L with within-run precision (CV) <2% from 1-10 g/L.
    • Interference from bilirubin, hemoglobin, and chylomicrons was eliminated.
    • Major interference from monoclonal proteins in adult samples was found to be very low (P = 0.0002).
    • Extremely high correlations were observed with immunoturbidimetric (r=0.99), chronometric (r=0.99), and clotting (r=0.97) methods.

    Conclusions:

    • The developed turbidimetric assay provides a reliable, precise, and accurate method for plasma fibrinogen measurement.
    • The assay is robust against common interfering substances and monoclonal proteins.
    • This method offers a valuable alternative for routine clinical laboratory use in assessing fibrinogen levels.