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Related Concept Videos

Labeling DNA Probes03:31

Labeling DNA Probes

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
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RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Related Experiment Video

Updated: Feb 18, 2026

Split Hybridization Probe Utilizing a DNA Fluorescent Light-up Aptamer as a Signal Reporter for Sequence-Specific Nucleic Acid Analysis
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Charged probes: turn-on selective fluorescence for RNA.

Bahareh Shirinfar1, Humaira Seema, Nisar Ahmed

  • 1School of Chemistry, University of Bristol, Cantock's Close, Bristol BS8 1TS, UK. shirinpostech@gmail.com.

Organic & Biomolecular Chemistry
|November 17, 2017
PubMed
Summary

Positively charged imidazolium probes enable selective detection and imaging of RNA. This advancement aids in investigating RNA

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Cellular Imaging

Background:

  • RNA is crucial for numerous biological processes.
  • Understanding RNA's cellular roles requires precise detection and imaging.
  • Current methods may have limitations in RNA specificity and visualization.

Purpose of the Study:

  • To develop and evaluate novel imidazolium-based probes for selective RNA detection.
  • To demonstrate the utility of these probes for cellular RNA imaging.
  • To explore the potential of these probes in molecular and drug design.

Main Methods:

  • Synthesis of positively charged imidazolium compounds.
  • In vitro and in cellulo testing for RNA binding specificity.
  • Fluorescence microscopy for cellular RNA imaging.

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Fluorescence Based Primer Extension Technique to Determine Transcriptional Starting Points and Cleavage Sites of RNases In Vivo
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Main Results:

  • Imidazolium probes exhibit high selectivity for RNA detection over DNA.
  • Successful imaging of RNA localization and dynamics within cells.
  • Demonstrated potential for probing RNA therapeutic targets.

Conclusions:

  • Positively charged imidazolium probes offer a promising tool for RNA research.
  • These probes facilitate the investigation of RNA's biological functions.
  • The technology supports future advancements in rational drug design for RNA-related diseases.