Saccharopolyspora aidingensis sp. nov., an actinomycete isolated from a salt lake

A novel halophilic actinomycete strain, designated TRM 46074^T, was isolated from Aiding salt lake in Turpan Basin, north-west China. Strain TRM 46074^T was aerobic, Gram-stain-positive and grew optimally with 12 % (w/v) NaCl. The strain was observed to produce sparse aerial mycelium with long chains of spores that were non-motile and oval or spherical in shape with a rough or smooth surface; substrate mycelium was branched and well developed. The cell wall was determined to contain meso-diaminopimelic acid; the diagnostic whole-cell sugars were galactose, arabinose and ribose. The diagnostic polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, phosphatidylglycerol and phosphatidylinositol. The predominant menaquinones were MK-9(H4), MK-10(H8) and MK-10(H4). The G+C content of the DNA was 70.9 mol%. The major fatty acids were anteiso-C17 : 0, iso-C15 : 0, iso-C16 : 0 and iso-C17 : 0. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain TRM 46074^T belongs to the genus Saccharopolyspora. Strain TRM 46074^T had 16S rRNA gene sequence similarity of 99.6 % with the closest described species, Saccharopolyspora ghardaiensis H53^T. DNA-DNA hybridization between strain TRM 46074^T and Saccharopolysporaghardaiensis H53^T showed 58.6 % relatedness. On the basis of the evidence from a polyphasic study, strain TRM 46074^T is considered to represent a novel species in the genus Saccharopolyspora, for which the name Saccharopolyspora aidingensis sp. nov. is proposed. The type strain is TRM 46074^T (=CCTCC AA 2012014^T=JCM 30185^T).