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Developing a Continuous Bioprocessing Approach to Stromal Cell Manufacture
Martina Miotto1, Ricardo Gouveia1, Fadhilah Zainal Abidin1
1Institute of Genetic Medicine, Newcastle University , International Centre for Life, Central Parkway, Newcastle-upon-Tyne NE1 3BZ, United Kingdom.
ACS Applied Materials & Interfaces
|November 18, 2017
Summary
This study introduces a novel continuous bioprocessing method for human adherent cells, utilizing a smart surface coating for controlled cell detachment and proliferation. This innovation enables high-yield, sustained cell sourcing for therapeutic applications.
Area of Science:
- Biotechnology
- Cell Biology
- Bioprocessing Engineering
Background:
- Continuous bioprocessing is primarily used for molecular biologics.
- Sourcing large numbers of human adherent cells for therapy remains challenging.
Purpose of the Study:
- To explore continuous bioprocessing for human adherent cells.
- To develop a method for high-throughput, standardized cell manufacturing.
Main Methods:
- Developed a smart multifunctional surface coating for human corneal stromal cells.
- Controlled cell attachment, proliferation, and self-detachment.
- Maintained steady-state cell culture conditions for continuous recovery.
Main Results:
- Achieved continuous recovery of ~1% of adherent cells per hour for 1 month.
- Demonstrated sustained cell phenotype in both attached and detached cells.
- Established a closed, continuous bioprocessing platform.
Conclusions:
- Proof-of-concept for continuous bioprocessing of human adherent cells.
- Potential for high-throughput, high-standard, low-cost biomanufacturing.
- Opens avenues for downstream therapeutic applications.
