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Updated: Feb 18, 2026

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

Published on: September 10, 2009

A Microfluidic Platform for the Characterisation of CNS Active Compounds.

Christopher MacKerron¹, Graham Robertson¹, Michele Zagnoni²

¹Centre for Microsystems and Photonics, Electronic and Electrical Engineering, University of Strathclyde, Glasgow, G1 1XW, UK.

Scientific Reports

|November 18, 2017

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This study introduces a novel microfluidic system for assessing central nervous system (CNS) compounds. The technology enables precise measurement of neuronal excitability and synaptic activity, aiding drug discovery.

Area of Science:

Neuroscience
Pharmacology
Biotechnology

Background:

Characterizing central nervous system (CNS) compounds requires advanced in vitro methods to assess neuronal excitability and synaptic activity.
Existing methodologies may lack the precision and control needed for comprehensive pharmacological profiling.

Purpose of the Study:

To present a novel microfluidic system for the pharmacological profiling of CNS-acting compounds.
To demonstrate the system's capability in measuring neuronal excitability and synaptic activity using calcium imaging.

Main Methods:

Development of a microfluidic system with computer-controlled compound perfusion.
Utilizing calcium imaging to monitor intracellular calcium changes in response to compound application.
Application of glutamate and glutamatergic receptor antagonists to validate the system's functionality.

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Last Updated: Feb 18, 2026

A Multi-compartment CNS Neuron-glia Co-culture Microfluidic Platform

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Published on: September 10, 2009

Real-Time Dynamic Collection of Hippocampal Extracellular Fluid from Conscious Rats Using a Microdialysis System

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Main Results:

The system generated reproducible concentration-response curves for glutamate, demonstrating its ability to assess neuronal excitability.
Glutamatergic receptor antagonists effectively inhibited glutamate-induced calcium increases, validating pharmacological profiling capabilities.
Repeated glutamate applications enhanced synaptically driven neuronal network activation, indicating assessment of synaptic connectivity.

Conclusions:

The presented microfluidic system offers a robust platform for neuropharmacological analysis of CNS compounds.
This technology enables simultaneous evaluation of compound effects on neuronal excitability and synaptic connectivity.
The methodology facilitates precise characterization of drugs targeting the central nervous system.