Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

14.6K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
14.6K
NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences01:17

NMR Spectrometers: Radiofrequency Pulses and Pulse Sequences

1.8K
A pulse is a short burst of radio waves distributed over a range of frequencies that simultaneously excites all the nuclei in the sample. Upon passing a radio frequency pulse along the x-axis, the nuclei absorb energy corresponding to their Larmor frequencies and achieve resonance. This shifts the net magnetization vector from the z-axis toward the transverse plane. This angle of rotation of the magnetization vector, or the flip angle, is proportional to the duration and intensity of the pulse.
1.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dual Quantum Dot Molecular FRET Probes for Picomolar DNA Hexaplexing.

Small methods·2026
Same author

Room-Temperature Luminescence of Eosin Y and Phloxine B in Red- to Near-Infrared Optical Region.

Journal of fluorescence·2026
Same author

Luminescence of N<sup>2</sup>,3-etheno-2-aminopurine Embedded in Polyvinyl Alcohol Films at Room Temperature.

Luminescence : the journal of biological and chemical luminescence·2026
Same author

Breaking the limit - synthesis and properties of <i>p</i>-amino-triazatriangulenium, the most stable carbenium ion.

Chemical science·2026
Same author

Twisting Rhodamine─Design of Bright Dyes for Circularly Polarized Fluorescence.

Journal of the American Chemical Society·2026
Same author

Room temperature luminescence of a triangulenium dye ADOTA in PVA films.

Methods and applications in fluorescence·2026
Same journal

Unsupervised clustering FLIM-phasor from multifunctionalized nanoparticles in living cancer cells.

Methods and applications in fluorescence·2026
Same journal

Resonance acoustic mixing-enabled non-covalent loading of Cy7 for enhanced solubility, stability, and fluorescent imaging performance.

Methods and applications in fluorescence·2026
Same journal

Substituent position-dependent photophysics in fluorinated porphycenes.

Methods and applications in fluorescence·2026
Same journal

Selective luminescence enhancement of lanthanide complexes using diffraction gratings.

Methods and applications in fluorescence·2026
Same journal

Fluorescence-based trace detection of explosives: current state and perspective for ultrasensitive portable technique via plasmonic enhancement.

Methods and applications in fluorescence·2026
Same journal

Compensating for photon counting losses in a TCSPC SPAD array enables quantitative time-resolved fluorescence anisotropy imaging.

Methods and applications in fluorescence·2026
See all related articles

Related Experiment Video

Updated: Feb 18, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

11.4K

Generating multiple-pulse bursts for enhanced fluorescence detection.

Dmytro Shumilov1, Ryan M Rich, Ignacy Gryczynski

  • 1Department of Physics & Astronomy, Texas Christian University, Fort Worth, TX 76129, USA.

Methods and Applications in Fluorescence
|November 18, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a new pulsed laser method to significantly improve fluorescence detection sensitivity. This technique enhances signals from long-lived probes by using high-repetition-rate bursts, overcoming limitations of current electronics for better fluorescence imaging and sensing.

More Related Videos

Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.3K
Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.9K

Related Experiment Videos

Last Updated: Feb 18, 2026

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers
10:21

Multicolor Fluorescence Detection for Droplet Microfluidics Using Optical Fibers

Published on: May 5, 2016

11.4K
Conducting Multiple Imaging Modes with One Fluorescence Microscope
08:32

Conducting Multiple Imaging Modes with One Fluorescence Microscope

Published on: October 28, 2018

10.3K
Fluorescence detection methods for microfluidic droplet platforms
14:16

Fluorescence detection methods for microfluidic droplet platforms

Published on: December 10, 2011

22.9K

Area of Science:

  • Analytical Chemistry
  • Spectroscopy
  • Biophysics

Background:

  • Signal-to-background ratio is critical for fluorescence detection, limited by scattering and autofluorescence.
  • Long-lived probes with time-gated detection improve signal-to-background but often sacrifice probe signal or brightness.
  • Existing methods struggle to amplify signals from probes with intermediate lifetimes (e.g., 20 ns) due to electronic limitations in generating high-repetition-rate lasers.

Purpose of the Study:

  • To present novel approaches for generating high-repetition-rate pulse bursts from low-repetition sources without advanced electronics.
  • To enhance the signal of fluorescence probes with intermediate lifetimes (e.g., 20 ns) for improved detection sensitivity.
  • To demonstrate a tunable method applicable to various sensing formats, improving fluorophore photostability.

Main Methods:

  • Utilized a pulsed laser source with a low repetition rate to generate bursts of pulses.
  • Optimized pulse burst parameters, achieving tunable 'burst repetition rates' up to 330 MHz (2-10 pulses spaced 3 ns apart).
  • Applied time-gated detection principles to differentiate long-lived probe signals from short-lived background noise.

Main Results:

  • Achieved significant signal enhancement for a 20 ns fluorescence lifetime probe.
  • Demonstrated effective suppression of sub-nanosecond/nanosecond background noise.
  • Showcased improved fluorophore photostability due to energy spreading across multiple pulses within a burst.

Conclusions:

  • The developed pulse burst technique offers a versatile and electronics-independent method for enhancing fluorescence detection sensitivity.
  • This approach enables higher sensitivity in various sensing applications by improving the signal-to-background ratio.
  • The method is tunable and enhances probe performance, including photostability, making it valuable for advanced fluorescence imaging and sensing.