Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

1.6K
Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
1.6K
Photoluminescence: Applications01:14

Photoluminescence: Applications

1.1K
Photoluminescence offers a wide range of applications due to its inherent sensitivity and selectivity. This technique allows for both direct and indirect analyses of the analyte. Direct quantitative analysis is possible when the analyte exhibits a favorable quantum yield for fluorescence or phosphorescence. However, an indirect analysis may be feasible if the analyte is not fluorescent or phosphorescent, or if the quantum yield is unfavorable. Indirect methods include reacting the analyte with...
1.1K
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

13.5K
Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.
13.5K
Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

21.3K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
21.3K
UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

3.0K
In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this...
3.0K
Flame Photometry: Overview01:02

Flame Photometry: Overview

1.6K
Flame photometry, also known as flame emission spectrometry, is a technique used for the qualitative and quantitative analysis of elements present in a sample using a flame as the source of excitation energy. The concept of flame photometry was realized in the early 1860s by Kirchhoff and Bunsen, who discovered that specific elements emit characteristic radiation when excited in flames. The first instrument developed for this purpose was used to measure sodium (Na) in plant ash using a Bunsen...
1.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Chirality Transfer, Memory and Sensing Activated by a Supramolecular Chiral Auxiliary Approach in Nanostructured, Tautomerically Prochiral Triptycene-Fused Benzimidazoles.

Journal of the American Chemical Society·2026
Same author

Monitoring the Switching from Base-on to Base-off Forms of Vitamin B<sub>12</sub> by Natural and Magnetic Circular Dichroism Spectroscopies.

Analytical chemistry·2026
Same author

Tuning linear dichroism/birefringence (LDLB) contributions in VCD spectra of tartaric acid-TPPS<sub>4</sub> porphyrin films.

Physical chemistry chemical physics : PCCP·2025
Same author

Circularly Polarized Luminescence from an Inverted Singlet-Triplet Chiral Dye.

Journal of the American Chemical Society·2025
Same author

Enhancement of the Chiroptical Properties of <i>o</i>-OPE through Arene-Perfluoroarene Interactions.

Organic letters·2025
Same author

The Role of Anharmonicity in the HC*D Chromophore in Vibrational Circular Dichroism Spectra and Optical Rotation Data.

The journal of physical chemistry. A·2025

Related Experiment Video

Updated: Feb 18, 2026

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

8.0K

Chiroptical spectroscopic techniques based on fluorescence.

Ettore Castiglioni1, Sergio Abbate, France Lebon

  • 1JASCO Europe, Cremella (LC), Italy. Dipartimento di Medicina Molecolare e Traslazionale, Università di Brescia, Brescia, Italy.

Methods and Applications in Fluorescence
|November 18, 2017
PubMed
Summary
This summary is machine-generated.

Chiroptical spectroscopic methods like circular dichroism (CD) and Raman optical activity (ROA) are established. Fluorescence-based techniques, including circularly polarized luminescence (CPL), are gaining traction, especially in material science.

More Related Videos

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
10:23

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Published on: April 25, 2025

1.1K
Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
06:08

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Published on: December 27, 2018

9.5K

Related Experiment Videos

Last Updated: Feb 18, 2026

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy
10:40

High Resolution Phonon-assisted Quasi-resonance Fluorescence Spectroscopy

Published on: June 28, 2016

8.0K
Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules
10:23

Time-Resolved Fluorescence Anisotropy from Single Molecules for Characterizing Local Flexibility in Biomolecules

Published on: April 25, 2025

1.1K
Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera
06:08

Time-resolved Photophysical Characterization of Triplet-harvesting Organic Compounds at an Oxygen-free Environment Using an iCCD Camera

Published on: December 27, 2018

9.5K

Area of Science:

  • Chiroptical spectroscopy
  • Photophysics
  • Material science

Background:

  • Established chiroptical methods include circular dichroism (CD), optical rotatory dispersion (ORD), and Raman optical activity (ROA).
  • Fluorescence-based chiroptical techniques, such as fluorescence detected circular dichroism and circularly polarized luminescence (CPL), have had limited applications historically.
  • The specialized hardware for CPL has restricted its user base.

Purpose of the Study:

  • To highlight the historical marginal role of fluorescence-based methods in chiroptical spectroscopy.
  • To discuss the recent surge in interest and new application fields for these techniques.
  • To emphasize the growing importance of emission properties in material science.

Main Methods:

  • Review of existing chiroptical spectroscopic techniques.
  • Discussion of fluorescence-detected circular dichroism and circularly polarized luminescence (CPL).
  • Analysis of factors driving recent interest, particularly in material science.

Main Results:

  • Fluorescence-based methods have historically played a minor role in chiroptical spectroscopy.
  • Circularly polarized luminescence (CPL) has faced limitations due to specialized hardware and a limited user base.
  • Renewed interest, driven by material science applications, is expanding the utility of these techniques.

Conclusions:

  • Fluorescence-based chiroptical methods, particularly CPL, are poised for broader application.
  • The increasing importance of emission properties in material science is a key driver for this shift.
  • Future developments may overcome previous limitations, increasing the accessibility and use of these techniques.