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Related Concept Videos

Bioreactor Controls-II01:18

Bioreactor Controls-II

In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...

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Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
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Imaging of oxygen in microreactors and microfluidic systems.

Shiwen Sun1, Birgit Ungerböck, Torsten Mayr

  • 1Applied Sensor Group, Institute of Analytical Chemistry, Graz University of Technology, Stremayrgasse 9, 8010 Graz, Austria.

Methods and Applications in Fluorescence
|November 18, 2017
PubMed
Summary

This review highlights oxygen imaging techniques for microfluidic systems. Luminescence-based optical oxygen sensors provide detailed spatial oxygen data for applications like cell culture and chemical reactions.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Microfluidics

Background:

  • Microfluidic systems require precise control and monitoring of oxygen levels.
  • Optical oxygen sensors offer a non-invasive method for oxygen measurement.
  • Luminescence-based sensing provides high spatial resolution for oxygen imaging.

Purpose of the Study:

  • To provide a comprehensive overview of the state-of-the-art in oxygen imaging for microfluidics.
  • To detail the principles, techniques, and applications of oxygen imaging in microfluidic devices.
  • To discuss current challenges and future trends in this field.

Main Methods:

  • Review of luminescence-based optical oxygen sensing principles.
  • Analysis of techniques for oxygen imaging in microreactors and microfluidic devices.
  • Discussion of selection criteria for sensing materials and system setup.

Main Results:

  • Oxygen imaging is a versatile tool for space-resolved oxygen information in microfluidics.
  • Applications include oxygen gradient monitoring, cell culturing, single-cell analysis, and chemical reactions.
  • Key aspects of sensing material selection and 2D oxygen sensing system setup are presented.

Conclusions:

  • Oxygen imaging using luminescence is crucial for advancing microfluidic research.
  • The technology enables detailed insights into oxygen dynamics in various microfluidic applications.
  • Further development is needed to address existing challenges and explore future trends.