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Fluorogen-based reporters for fluorescence imaging: a review
Ludovic Jullien1, Arnaud Gautier
1École Normale Supérieure-PSL Research University, Department of Chemistry, 24 rue Lhomond, F-75005 Paris, France. Sorbonne Universités, UPMC Univ Paris 06, UMR 8640 PASTEUR, F-75005 Paris, France. CNRS, UMR 8640 PASTEUR, F-75005 Paris, France.
Methods and Applications in Fluorescence
|November 18, 2017
Summary
Genetically encodable fluorescent reporters that bind fluorogenic chromophores offer enhanced spatiotemporal resolution and sensitivity in bioimaging. These innovative probes are advancing applications in cellular imaging and biosensing.
Area of Science:
- Biophysics
- Molecular Biology
- Optical Physics
Background:
- Fluorescence bioimaging has advanced significantly due to progress in optical physics and probe design.
- Genetically encodable reporters offer a powerful tool for in vivo imaging.
- Existing fluorescent reporters have limitations in sensitivity and spatiotemporal resolution.
Purpose of the Study:
- To review recent developments in genetically encodable fluorescent reporters.
- To highlight the design and engineering of novel fluorescent reporters.
- To describe emerging applications of these reporters in bioimaging and biosensing.
Main Methods:
- Review of scientific literature on fluorescent reporters and bioimaging techniques.
- Analysis of innovative engineering and design principles for fluorescent reporters.
- Synthesis of information on the binding of fluorogenic chromophores to reporters.
Main Results:
- Development of genetically encodable reporters that bind endogenous or exogenous fluorogenic chromophores.
- Activation of fluorescence upon binding of fluorogenic chromophores.
- Demonstration of enhanced spatiotemporal resolution and sensitivity in bioimaging.
Conclusions:
- Genetically encodable fluorescent reporters represent a significant advancement in bioimaging.
- These reporters enable new possibilities for in vivo imaging and biosensing.
- Continued innovation in probe design promises further breakthroughs in the field.
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