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Microfluidic Acoustophoresis for Flowthrough Separation of Gram-Negative Bacteria using Aptamer Affinity Beads
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Acoustic Wave-Driven Functionalized Particles for Aptamer-Based Target Biomolecule Separation.

Raheel Ahmad1, Ghulam Destgeer1, Muhammad Afzal2

  • 1Department of Mechanical Engineering, KAIST , 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Korea.

Analytical Chemistry
|November 18, 2017
PubMed
Summary
This summary is machine-generated.

This study introduces a novel acoustofluidic device for protein separation. The hybrid microfluidic system uses acoustic waves to isolate target proteins, like thrombin, from complex biological samples with high selectivity.

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Area of Science:

  • Biotechnology
  • Microfluidics
  • Biochemistry

Background:

  • Protein separation from complex biological fluids remains a significant challenge in diagnostics and research.
  • Existing methods often lack specificity or require extensive sample preparation.

Purpose of the Study:

  • To develop and validate a hybrid microfluidic device for selective protein separation using acoustic waves.
  • To demonstrate the capture and separation of a model target protein, thrombin, from complex biological matrices.

Main Methods:

  • A hybrid acoustofluidic device integrating an interdigitated transducer and a polydimethylsiloxane (PDMS) microchannel was designed.
  • Streptavidin-functionalized polystyrene (PS) microparticles conjugated with aptamer15 (apt15) were used to capture biotin-labeled target proteins.
  • Surface acoustic waves (SAW) were employed to deflect and separate particle-conjugated target proteins from non-target proteins in continuous flow.

Main Results:

  • The PS-apt15 conjugate selectively captured thrombin (th) molecules from complex fluids.
  • The acoustofluidic device successfully separated thrombin from mCardinal2 and human serum.
  • Hydrodynamic focusing enhanced the efficiency of SAW-based separation.

Conclusions:

  • The developed acoustofluidic device offers a promising platform for selective protein isolation.
  • This technology has potential applications in sensitive and specific biomarker detection.
  • The hybrid microfluidic system demonstrates efficient separation of target proteins from complex biological samples.