Related Experiment Video
Updated: Feb 18, 2026
Absorbent Microbiopsy Sampling and RNA Extraction for Minimally Invasive, Simultaneous Blood and Skin Analysis
Published on: February 21, 2019
Volumetric absorptive microsampling combined with impact-assisted extraction for hematocrit effect free assays
Nikolay Youhnovski1, Laurence Mayrand-Provencher1, Eugénie-Raphaëlle Bérubé1
1Altasciences Clinical Research, 575 Armand-Frappier, Laval, Quebec H7V 4B3, Canada.
Aim:
Volumetric absorptive microsampling (VAMS) is a recent technology available for sampling and analyzing low blood volume. The present work describes the utilization of VAMS for the quantitation of naproxen and ritonavir in human blood using a novel bead-based impact-assisted extraction (IAE) procedure.
Results:
Sampling volume accuracy of the VAMS device was independent of the blood hematocrit (HCT) level, however analyte recovery decreased with increasing HCT when extracted using ultrasonication. In contrast, IAE was unaffected by HCT, resulting in quantitative recovery for all levels evaluated. Precision and accuracy batches, as well as matrix effect evaluation, met acceptance criteria.
Conclusion:
The IAE procedure coupled with VAMS is immune to HCT biases affecting sampling volume and recovery.
