Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Maintenance of the ES Cell State01:14

Maintenance of the ES Cell State

2.8K
The cells of the blastocyst inner cell mass only remain pluripotent for a short time. This state of pluripotency and self-renewal can be maintained in embryonic stem (ES) cell culture by adding specific chemicals or growth factors to ensure the cells can continue dividing and later differentiate into different cell types. In some cases, the cells are grown on a feeder layer of differentiated cells, which provides the growth factors and extracellular matrix components necessary for stem cell...
2.8K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

2.7K
Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.7K
S-Cdk Initiates DNA Replication02:38

S-Cdk Initiates DNA Replication

5.7K
The cell cycle is a series of events leading to DNA duplication followed by the division of cell content to form two daughter cells. The cell cycle progresses in four stages—the cell increases in size (gap 1 or G1-phase), duplicates its DNA (synthesis or S-phase), prepares to divide (gap 2 or G2-phase), and divides (mitosis or M-phase).
Two states at the origin of replication
In eukaryotes, the initiation of replication occurs at many sites on the chromosomes, called the origins of...
5.7K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

5.7K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.7K
Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

28.2K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
28.2K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

2.2K
Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The RNA binding protein ZFP36L2 displays tissue-selective mRNA targeting in mice.

RNA biology·2026
Same author

Occurrence patterns of Myllocerinus aurolineatus (Coleoptera: Curculionidae) and histological observation of adult female tissues.

Environmental entomology·2026
Same author

Electrochemical α-Arylation of Quinolinones with Site-Selective Dehydrogenative Coupling.

Organic letters·2026
Same author

Non-Genetic Mechanisms of Fractional Resistance to Abemaciclib in Dedifferentiated Liposarcoma.

bioRxiv : the preprint server for biology·2026
Same author

Efficient, fratricide free non-viral engineering of CD70-targeted CAR NK cells for hematologic and solid tumor immunotherapy.

Molecular therapy. Advances·2026
Same author

Extrachromosomal DNA Gives Cancer a New Evolutionary Pathway.

Research square·2026
Same journal

The exquisite mechanics of a tsetse bite.

eLife·2026
Same journal

Distinct involvements of the subthalamic nucleus subpopulations in reward-biased decision-making in monkeys.

eLife·2026
Same journal

Pink1-mediated mitophagy in the endothelium releases proteins encoded by mitochondrial DNA and activates neutrophil responses during inflammation.

eLife·2026
Same journal

Restraint of melanoma progression by cells in the local skin environment.

eLife·2026
Same journal

Brawn before bite in endemic Asian eutherian mammals after the end-Cretaceous extinction.

eLife·2026
Same journal

Experimental evolution to thermal stress indicates climate resilience in a cosmopolitan arthropod.

eLife·2026
See all related articles

Related Experiment Video

Updated: Feb 18, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.9K

Rapid DNA replication origin licensing protects stem cell pluripotency.

Jacob Peter Matson1, Raluca Dumitru2, Philip Coryell3

  • 1Department of Biochemistry and Biophysics, The University of North Carolina, Chapel Hill, United States.

Elife
|November 18, 2017
PubMed
Summary
This summary is machine-generated.

Pluripotent stem cells rapidly license DNA replication origins due to high Cdt1 levels, a process crucial for maintaining pluripotency. Slowing this origin licensing accelerates differentiation and lengthens the G1 phase.

Keywords:
Cdt1G1MCMcell cyclechromosomesdifferentiationgeneshumansingle cell analysis

More Related Videos

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells
12:06

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Published on: January 11, 2019

13.1K
Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor
07:28

Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor

Published on: February 1, 2015

20.6K

Related Experiment Videos

Last Updated: Feb 18, 2026

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal
08:01

Oct4GiP Reporter Assay to Study Genes that Regulate Mouse Embryonic Stem Cell Maintenance and Self-renewal

Published on: May 30, 2012

10.9K
Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells
12:06

Generation of Human Primordial Germ Cell-like Cells at the Surface of Embryoid Bodies from Primed-pluripotency Induced Pluripotent Stem Cells

Published on: January 11, 2019

13.1K
Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor
07:28

Transfection, Selection, and Colony-picking of Human Induced Pluripotent Stem Cells TALEN-targeted with a GFP Gene into the AAVS1 Safe Harbor

Published on: February 1, 2015

20.6K

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • DNA replication origin licensing, involving minichromosome maintenance (MCM) helicase loading, is essential for genome duplication.
  • Origin licensing occurs during the G1 phase of the cell cycle, whose duration varies significantly across cell types.

Purpose of the Study:

  • To investigate the relationship between G1 phase length and MCM loading kinetics in pluripotent stem cells versus differentiated cells.
  • To elucidate the molecular mechanisms underlying rapid origin licensing in stem cells and its role in maintaining pluripotency.

Main Methods:

  • Quantitative single-cell analyses were employed to measure MCM loading rates.
  • Cellular differentiation was induced, and the effects of ectopic Cyclin E were examined.

Main Results:

  • Pluripotent stem cells with short G1 phases exhibit significantly faster MCM loading than differentiated cells with longer G1 phases.
  • MCM loading slows during early differentiation, correlating with G1 lengthening, indicating developmental control.
  • Rapid MCM loading in stem cells is attributed to the accumulation of the Cdt1 protein.
  • Accelerated differentiation and G1 lengthening occur when MCM loading is prematurely slowed in pluripotent cells.

Conclusions:

  • Rapid origin licensing is an intrinsic characteristic of stem cells, contributing to pluripotency maintenance.
  • Developmental control of G1 phase length and MCM loading are tightly linked.
  • Cdt1 accumulation is a key factor driving rapid MCM loading in stem cells.