Revealing the distribution of transmembrane currents along the dendritic tree of a neuron from extracellular...

Revealing the current source distribution along the neuronal membrane is a key step on the way to understanding neural computations; however, the experimental and theoretical tools to achieve sufficient spatiotemporal resolution for the estimation remain to be established. Here, we address this problem using extracellularly recorded potentials with arbitrarily distributed electrodes for a neuron of known morphology. We use simulations of models with varying complexity to validate the proposed method and to give recommendations for experimental applications. The method is applied to in vitro data from rat hippocampus.