Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Classification of Neurotransmitters01:30

Classification of Neurotransmitters

Neurotransmitters play a crucial role in the communication between neurons in the autonomic nervous system. Neurons in the autonomic nervous system can be cholinergic or adrenergic depending on the neurotransmitters synthesized. Cholinergic neurons use acetylcholine as their primary neurotransmitter. This includes all the preganglionic fibers of the sympathetic and pre- and postganglionic fibers of the parasympathetic nervous systems. In addition, neurons of the somatic nervous system also use...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Transformation of dissolved organic matter during full-scale treatment of integrated chemical wastewater: Molecular composition correlated with spectral indexes and acute toxicity.

Water research·2019
Same author

Epicardial ganglionated plexi ablation increases the inducibility of ventricular tachyarrhythmias in a canine postmyocardial infarction model.

Journal of cardiovascular electrophysiology·2019
Same author

Value of <sup>18</sup>F-FDG PET/CT in the diagnosis of portal vein tumor thrombus in patients with hepatocellular carcinoma.

Abdominal radiology (New York)·2019
Same author

Accurate Determination of Trace Molybdenum in Drinking Water by Isotope Dilution Inductively Coupled Plasma Mass Spectrometry.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2019
Same author

Using clinical genomic sequencing to guide personalized cancer therapy in China.

Personalized medicine·2019
Same author

Value of High-Resolution DWI in Combination With Texture Analysis for the Evaluation of Tumor Response After Preoperative Chemoradiotherapy for Locally Advanced Rectal Cancer.

AJR. American journal of roentgenology·2019

Related Experiment Video

Updated: Jun 14, 2026

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
11:26

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

14.4K

Classifying Drosophila Olfactory Projection Neuron Subtypes by Single-Cell RNA Sequencing.

Hongjie Li1, Felix Horns2, Bing Wu1

  • 1Department of Biology and Howard Hughes Medical Institute, Stanford University, Stanford, CA 94305, USA.

Cell
|November 18, 2017
PubMed
Summary

Neuronal transcriptomic identity in Drosophila olfactory projection neurons (PNs) changes during development. Gene expression differences are greatest in developing PNs, highlighting developmental stages for neuronal diversity.

Keywords:
DrosophilaSingle-cell RNA-seqcell typecombinatorial codeconnectivitydevelopmentneuronal identityolfactory systemprojection neurontranscriptome

More Related Videos

Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons
06:49

Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons

Published on: March 21, 2018

7.9K
Preparing Developing Peripheral Olfactory Tissue for Molecular and Immunohistochemical Analysis in Drosophila
09:18

Preparing Developing Peripheral Olfactory Tissue for Molecular and Immunohistochemical Analysis in Drosophila

Published on: June 13, 2018

6.2K

Related Experiment Videos

Last Updated: Jun 14, 2026

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells
11:26

Single-cell RNA-Seq of Defined Subsets of Retinal Ganglion Cells

Published on: May 22, 2017

14.4K
Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons
06:49

Tracking Drosophila Larval Behavior in Response to Optogenetic Stimulation of Olfactory Neurons

Published on: March 21, 2018

7.9K
Preparing Developing Peripheral Olfactory Tissue for Molecular and Immunohistochemical Analysis in Drosophila
09:18

Preparing Developing Peripheral Olfactory Tissue for Molecular and Immunohistochemical Analysis in Drosophila

Published on: June 13, 2018

6.2K

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Genomics

Background:

  • Defining neuronal types and their relationship to gene expression (transcriptome) remains a challenge.
  • Drosophila olfactory projection neurons (PNs) are well-defined neuronal types with distinct connectivity and function.
  • Understanding transcriptomic identity is crucial for characterizing neuronal subtypes.

Purpose of the Study:

  • To comprehensively characterize the transcriptomes of Drosophila olfactory projection neuron (PN) classes.
  • To map transcriptomes to specific olfactory functions.
  • To investigate the dynamic changes in transcriptomic identity during neuronal development.

Main Methods:

  • Single-cell RNA sequencing was employed to analyze transcriptomes of most PN classes.
  • Transcriptomic data was mapped to specific olfactory functions for six PN classes.
  • Differential gene expression analysis was performed to identify key molecular markers.

Main Results:

  • Transcriptomes of closely related PN classes show significant differences during development, becoming indistinguishable in adults.
  • Neuronal subtype diversity peaks during the developmental stages of circuit assembly.
  • Transcription factors and cell-surface molecules are key differentially expressed genes encoding cell identity.
  • A novel lineage-specific transcription factor involved in PN dendrite targeting was identified.

Conclusions:

  • Neuronal transcriptomic identity aligns with anatomical and physiological identity defined by connectivity and function.
  • Transcriptomic analysis provides a powerful tool for understanding neuronal diversity and development.
  • The findings suggest that developmental timing is critical for establishing neuronal subtype specificity.