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CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

Homologous Recombination

Homologous Recombination

The basic reaction of homologous recombination (HR) involves two chromatids that contain DNA sequences sharing a significant stretch of identity. One of these sequences uses a strand from another as a template to synthesize DNA in an enzyme-catalyzed reaction. The final product is a novel amalgamation of the two substrates. To ensure an accurate recombination of sequences, HR is restricted to the S and G2 phases of the cell cycle. At these stages, the DNA has been replicated already and the...

What is Genetic Engineering?

What is Genetic Engineering?

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Related Experiment Video

Updated: Feb 18, 2026

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

Published on: January 5, 2018

CRISPR/Cas9 Technology: Applications and Human Disease Modeling.

Marta Martinez-Lage¹, Raúl Torres-Ruiz¹, Sandra Rodriguez-Perales¹

¹Molecular Cytogenetics and Genome Engineering Group, Centro Nacional Investigaciones Oncológicas (CNIO), Madrid, Spain.

Progress in Molecular Biology and Translational Science

|November 19, 2017

Summary

The CRISPR/Cas9 system enables precise DNA editing, revolutionizing genome engineering for biomedical research. This technology expands gene function studies and disease modeling, promising future medical advancements.

Keywords:

CRISPR applications activation disease model gene editing genome engineering repression

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Published on: August 25, 2017

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Published on: August 2, 2018

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Using CRISPR/Cas9 Gene Editing to Investigate the Oncogenic Activity of Mutant Calreticulin in Cytokine Dependent Hematopoietic Cells

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Published on: January 5, 2018

A Standard Methodology to Examine On-site Mutagenicity As a Function of Point Mutation Repair Catalyzed by CRISPR/Cas9 and SsODN in Human Cells

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Published on: August 25, 2017

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Adeno-Associated Virus-Mediated Delivery of CRISPR for Cardiac Gene Editing in Mice

Published on: August 2, 2018

Area of Science:

Genomics
Molecular Biology
Biotechnology

Background:

The CRISPR/Cas9 system has emerged as a powerful tool for genome engineering.
It allows for targeted DNA modifications in various organisms and cell types.
Its applications extend beyond simple gene editing.

Purpose of the Study:

To highlight the revolutionary impact of CRISPR/Cas9 in genome engineering.
To outline the diverse applications of CRISPR/Cas9 technology.
To discuss the potential of CRISPR/Cas9 in advancing biomedical research and medicine.

Main Methods:

CRISPR/Cas9-mediated targeted DNA cleavage.
Gene editing techniques (knock-in/knock-out).
Applications in gene expression regulation, epigenome editing, and RNA editing.

Main Results:

CRISPR/Cas9 facilitates efficient and targeted DNA modifications.
The system supports a broad spectrum of applications including gene regulation and epigenome editing.
It enhances the study of gene function and the development of disease models.

Conclusions:

CRISPR/Cas9 technology has significantly advanced genome engineering capabilities.
Its diverse applications are crucial for biomedical research and developing new medical treatments.
Further research addressing mechanistic questions and challenges will enhance its future clinical utility.