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Related Concept Videos

Genetic Screens02:46

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Genetic screens are tools used to identify genes and mutations responsible for phenotypes of interest. Genetic screens help identify individuals or a group of people at risk of developing  genetic diseases and help them with early intervention, targeted therapy, and reproductive options.
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Updated: Feb 18, 2026

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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CRISPR Libraries and Screening.

John T Poirier1

  • 1Department of Medicine, Memorial Sloan Kettering Cancer Center, New York, NY, United States.

Progress in Molecular Biology and Translational Science
|November 19, 2017
PubMed
Summary
This summary is machine-generated.

CRISPR-Cas9 technology enables functional genomic screening in animals, moving beyond cell cultures. This approach uses lentiviral vectors for in vivo studies, particularly in mouse cancer models, offering more relevant biological insights.

Keywords:
CRISPRCas9animal modelscancerin vivoscreen

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Area of Science:

  • Genomics
  • Molecular Biology
  • Cancer Research

Background:

  • CRISPR-Cas9 technology has transformed functional genomic screening in cell cultures.
  • Lentiviral delivery vectors facilitate the expansion of these screens into animal models.
  • In vivo screening provides a more physiologically relevant context for studying biological processes.

Purpose of the Study:

  • To review the application of CRISPR-Cas9 for functional genomic screening in animal models.
  • To discuss viral vectors for simultaneous tumor initiation and genome editing in mice.
  • To explore considerations for single-guide RNA (sgRNA) library design in vivo.

Main Methods:

  • Utilizing lentiviral vectors for CRISPR-Cas9 delivery in vivo.
  • Employing mouse models of human cancers for functional screening.
  • Analyzing existing literature on in vivo RNAi screens.

Main Results:

  • CRISPR-Cas9 screens are now feasible in animals, enhancing biological relevance.
  • Lentiviral vectors are key for simultaneous tumor initiation and genome editing.
  • Challenges exist in delivering diverse small RNA libraries in vivo.

Conclusions:

  • In vivo CRISPR-Cas9 screening offers significant advantages over cell-culture systems.
  • Optimized viral vectors and library design are crucial for successful in vivo screens.
  • Further research is needed to overcome delivery challenges for small RNA libraries in vivo.