Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

From plasmid sequence to process design: A computational analysis of metabolism in the context of plasmid DNA manufacturing.

Journal of biotechnology·2026
Same author

3D Bioprinting of High-Grade Serous Ovarian Cancer Cells: Workflow for Gelatin-Based Bioink Formulation with Hyaluronic Acid and Printability Assessment.

ACS applied bio materials·2026
Same author

Suicide of the Work-Disabled Individual Affected by Neoplasia with Unfavorable Prognosis: Evidence-Based Legal Medicine in Reconstructing the Causal Link.

La Clinica terapeutica·2026
Same author

Corrigendum to 'Biosynthesis, characterisation and biocompatibility of a unique and elastomeric medium chain-length polyhydroxyalkanoate for kidney glomerular tissue engineering' [Mater. Today Bio 33 (2025), 101932].

Materials today. Bio·2026
Same author

On the Prediction of pDNA Productivity Across Diverse Bioprocesses Using Ensemble Hybrid Models.

Biotechnology and bioengineering·2026
Same author

Metal-Coordinated His-Tag Functionalization of Polymeric Nanogels for Therapeutic Applications.

ACS applied nano materials·2026

Related Experiment Video

Updated: Feb 18, 2026

Author Spotlight: Advancing Metabolomics Analysis of Rare Hematopoietic Stem Cells
08:28

Author Spotlight: Advancing Metabolomics Analysis of Rare Hematopoietic Stem Cells

Published on: February 23, 2024

2.6K

NMR Metabolomics for Stem Cell type discrimination.

Franca Castiglione1, Monica Ferro1, Evangelos Mavroudakis1,2

  • 1Department of Chemistry Materials and Chemical Engineering "G. Natta", Politecnico di Milano, Milan, Italy.

Scientific Reports
|November 19, 2017
PubMed
Summary
This summary is machine-generated.

Cell metabolism significantly impacts stem cell (SC) function. This study reveals distinct metabolic profiles for Neural SCs (NSCs) and Olfactory Ensheathing Cells (OECs), differentiating them by age and type.

More Related Videos

Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry
07:57

Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

Published on: December 26, 2019

13.0K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

7.1K

Related Experiment Videos

Last Updated: Feb 18, 2026

Author Spotlight: Advancing Metabolomics Analysis of Rare Hematopoietic Stem Cells
08:28

Author Spotlight: Advancing Metabolomics Analysis of Rare Hematopoietic Stem Cells

Published on: February 23, 2024

2.6K
Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry
07:57

Measurement of Mitochondrial Mass and Membrane Potential in Hematopoietic Stem Cells and T-cells by Flow Cytometry

Published on: December 26, 2019

13.0K
A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations
09:34

A Combinatorial Single-cell Approach to Characterize the Molecular and Immunophenotypic Heterogeneity of Human Stem and Progenitor Populations

Published on: October 25, 2018

7.1K

Area of Science:

  • Stem cell biology
  • Metabolomics
  • Neuroscience

Background:

  • Cell metabolism is crucial for stem cell (SC) pluripotency and fate.
  • Understanding SC metabolic heterogeneity is key for development, aging, and disease.
  • Neural SCs (NSCs) and Olfactory Ensheathing Cells (OECs) are critical glial progenitors.

Purpose of the Study:

  • To characterize and compare the metabolic profiles of different SC subpopulations.
  • To identify metabolic fingerprints distinguishing NSCs and OECs.
  • To investigate age-related metabolic differences in NSCs.

Main Methods:

  • Isolation and culture of rodent fetal, postnatal, and adult NSCs from the SVZ.
  • Isolation and culture of postnatal OECs from the OB.
  • Metabolite identification and quantification using proton Nuclear Magnetic Resonance (1H-NMR) spectroscopy.
  • Multivariate data analysis including PCA and PLS-DA for cluster analysis.

Main Results:

  • Distinct metabolic fingerprints were identified for different SC types.
  • A clear metabolic divergence was observed between OECs and NSCs.
  • Statistically significant metabolic differences were found in NSCs of varying ages.
  • Retrieved metabolomes correlated with physiological SC features.

Conclusions:

  • Metabolic profiling provides specific molecular signatures for (neural) SCs.
  • This approach enables the dissection of SC molecular specificities.
  • Integrated bioengineering of biologic fingerprints can advance SC research.