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Isolation and Quantitative Immunocytochemical Characterization of Primary Myogenic Cells and Fibroblasts from Human Skeletal Muscle
Published on: January 12, 2015
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Isolation of Primary Human Skeletal Muscle Cells
Janelle M Spinazzola1,2, Emanuela Gussoni1,2,3
1Division of Genetics and Genomics, Boston Children's Hospital, Boston, MA, USA.
Bio-Protocol
|November 21, 2017
Abstract
None:
Primary myoblast culture is a valuable tool in research of muscle disease, pathophysiology, and pharmacology. This protocol describes techniques for dissociation of cells from human skeletal muscle biopsies and enrichment for a highly myogenic population by fluorescence-activated cell sorting (FACS). We also describe methods for assessing myogenicity and population expansion for subsequent in vitro study.
