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Updated: Feb 18, 2026

Fluorescence-based Monitoring of PAD4 Activity via a Pro-fluorescence Substrate Analog
Published on: November 5, 2014
Fluorenone based fluorescent probe for selective "turn-on" detection of pyrophosphate and alanine
Daniel Thangadurai T1, Nithya I1, Manjubaashini N1
1Department of Nanoscience and Technology, Sri Ramakrishana Engineering College, Coimbatore 641 022, Tamilnadu, India.
Abstract:
To sense biologically important entities with different size and dimensions, a fluorenone based fluorescent receptor was designed and synthesized. Probe 1 displayed a distinct fluorescence enhancement emission at 565nm for pyrophosphate and 530nm for alanine in polar solvent. The fluorescence titration experiments confirm 1:1 stoichiometric ratio with high-binding constant and very low limit of detection (LoD) values. Receptor 1 showed a highly selective and sensitive recognition to HP2O73- and to alanine over other competitive anions and amino acids. In addition, the fluorescence lifetime measurement and reversible binding study results support the practical importance of 1.
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