Related Experiment Videos
Cu@Pd core-shell nanostructures for highly sensitive and selective amperometric analysis of histamine
Rajendra Kumar Reddy Gajjala1, Suresh Kumar Palathedath1
1School of Chemical and Biotechnology, SASTRA University, Thanjavur, Tamilnadu 613401, India.
Biosensors & Bioelectronics
|November 21, 2017
Summary
We developed a stable and sensitive histamine sensor using copper-palladium core-shell nanostructures on pencil graphite. This cost-effective sensor offers precise detection at low potentials, outperforming existing methods.
Area of Science:
- Materials Science
- Electrochemistry
- Analytical Chemistry
Background:
- Developing sensitive and selective electrochemical sensors is crucial for detecting biogenic amines like histamine.
- Existing methods often suffer from high oxidation potentials, interference, and limited sensitivity.
Purpose of the Study:
- To create a facile and rapid methodology for preparing stable Cu@Pd core-shell nanostructures on a pencil graphite substrate.
- To utilize these nanostructures for sensitive and selective electrochemical sensing of histamine.
- To achieve a low oxidation potential and high sensitivity for histamine detection.
Main Methods:
- Fabrication of Cu@Pd core-shell nanostructures on a pencil graphite substrate via galvanic replacement reaction.
- Electrochemical characterization of the nanostructures.
- Histamine sensing using chronoamperometry at a low oxidation potential (+0.55V vs. Ag/AgCl).
- Selectivity testing against common interfering biogenic amines.
- Validation using High-Performance Liquid Chromatography (HPLC) in real samples.
Main Results:
- Successfully prepared stable Cu@Pd core-shell nanostructures with excellent electrocatalytic activity.
- Achieved sensitive and selective histamine detection at a low oxidation potential (+0.55V vs. Ag/AgCl), avoiding oxygen evolution interference.
- Demonstrated excellent selectivity towards histamine even in the presence of other biogenic amines.
- Obtained a high sensitivity of 0.082 μA/μM/cm² and a low limit of detection of 3.2 ± 0.1 nM.
- Validated the sensor's practical feasibility by analyzing histamine in canned tuna fish samples.
Conclusions:
- The developed Cu@Pd core-shell nanostructure sensor offers a superior alternative for histamine detection due to its low oxidation potential, high sensitivity, and selectivity.
- This cost-effective and stable sensor provides a promising platform for practical applications in food safety and biochemical analysis.
- The methodology represents a significant advancement over existing electrochemical sensing techniques for histamine.
Related Concept Videos
01:10Amperometry: Overview
1.8K
Amperometry is a technique commonly used to measure the concentration of specific analytes in a solution by monitoring the electric current generated during an electrochemical reaction. It involves applying a constant potential between a working electrode and a reference electrode to measure the resulting current, which is proportional to the concentration of the analyte. The Clark oxygen electrode operates based on this principle of amperometry. It consists of a cathode and an anode enclosed...
1.8K
01:15Potentiometry: Membrane Electrodes
1.9K
Membrane electrodes, also known as p-ion electrodes, use membranes that selectively interact with free analyte ions, generating a potential difference across the membrane. The resulting membrane potential, known as the asymmetry potential, is not zero even when analyte concentrations on both sides of the membrane are equal. The membrane's response is typically not selective to a single analyte but proportional to the concentration of all ions in the sample solution capable of interacting at...
1.9K
01:15High-Performance Liquid Chromatography: Types of Detectors
1.8K
The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte...
1.8K
01:18Patch Clamp
6.8K
Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...
6.8K
01:17Controlled-Potential Coulometry: Electrolytic Methods
752
Controlled-potential coulometry, also known as potentiostatic coulometry, employs a three-electrode system in which the working electrode's potential is precisely regulated using a potentiostat. Platinum working electrodes are utilized for positive potentials, while mercury pool electrodes are favored for extremely negative potentials. The platinum counter electrode is separated from the analyte using a membrane or salt bridge to avoid interference in the analysis.
The chosen potential...
The chosen potential...
752
01:19NMR Spectroscopy Of Amines
11.3K
In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is...
11.3K