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Related Experiment Video

Updated: Feb 18, 2026

Organoid-Derived Epithelial Monolayer: A Clinically Relevant In Vitro Model for Intestinal Barrier Function
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Development and Characterization of a Human and Mouse Intestinal Epithelial Cell Monolayer Platform.

Kenji Kozuka1, Ying He1, Samantha Koo-McCoy1

  • 1Ardelyx, Inc., 34175 Ardenwood Boulevard, Suite 200, Fremont, CA 94555, USA.

Stem Cell Reports
|November 21, 2017
PubMed
Summary

Researchers developed a novel intestinal epithelial cell monolayer model for drug discovery. This platform accurately mimics human and mouse small and large intestines, enabling the identification of new drug candidates.

Keywords:
coloncolonoidenteroidintestinal epitheliummonolayerorganoidsphenotype screening assayssmall intestine

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Area of Science:

  • Gastroenterology
  • Drug Discovery
  • Cell Biology

Background:

  • Developing in vitro models of the intestine is crucial for drug discovery.
  • Existing models often fail to replicate the complex physiology of different intestinal segments.

Purpose of the Study:

  • To create and characterize a robust mouse and human intestinal epithelial cell monolayer platform.
  • To enable the discovery of minimally systemic drug candidates.

Main Methods:

  • Optimized culture conditions by correlating gene expression between monolayers and native tissues.
  • Validated monolayer polarization, tight junction formation, and cell lineage diversity.
  • Assessed ion transport, secretion, and absorption functions.

Main Results:

  • Developed polarized intestinal monolayers with diverse cell lineages.
  • Monolayer ion transport phenotypes accurately reflected native intestinal segments.
  • Demonstrated intact secretory (serotonin, GLP-1, FGF19) and absorptive functions.
  • Identified a tool compound by screening over 2,000 compounds for potassium channel inhibition.

Conclusions:

  • The developed intestinal monolayer platform accurately models human and mouse intestinal physiology.
  • This platform is suitable for drug discovery, particularly for minimally systemic agents.
  • The study successfully identified a novel tool compound for further research.