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Improving the selectivity and sensitivity for quantifying 8-α-hydroxy-mutilin in rabbit tissues by using basic mobile

Aimin Tan1, Guifen Gu1, Xuan Gui1

  • 1Nucro-Technics, Scarborough, ON, Canada.

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
|November 21, 2017
PubMed
Summary

A new LC-MS/MS method improves quantification of 8-α-hydroxy-mutilin, a tiamulin residue marker, in rabbit tissues. This method uses a novel true MRM transition for enhanced selectivity and sensitivity in residue depletion studies.

Keywords:
8-α-hydroxy-mutilinLC–MSRabbitTiamulinTissue

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Area of Science:

  • Analytical Chemistry
  • Pharmacokinetics
  • Veterinary Drug Residue Analysis

Background:

  • Quantifying 8-α-hydroxy-mutilin, a tiamulin marker residue, in tissues is crucial for food safety.
  • Previous LC-MS methods suffered from suboptimal selectivity and sensitivity due to pseudo MRM transitions.

Purpose of the Study:

  • To develop and validate a highly sensitive and selective LC-MS/MS method for quantifying 8-α-hydroxy-mutilin in rabbit tissues.
  • To establish a reliable analytical method for supporting residue depletion studies.

Main Methods:

  • Utilized electrospray negative ionization in a basic medium to discover a true MRM transition (m/z 335→179).
  • Developed and validated a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method using pleuromutilin as an internal standard.
  • Quantified 8-α-hydroxy-mutilin in rabbit muscle, liver, kidney, and fat tissues.

Main Results:

  • A novel, highly selective and sensitive true MRM transition (m/z 335→179) was identified for 8-α-hydroxy-mutilin.
  • The developed LC-MS/MS method demonstrated significant improvements in selectivity and sensitivity compared to previous methods.
  • Validated method showed excellent accuracy (-5.0 to 3.1% bias) and precision (2.2 to 8.3% CV) within the 0.2-10 ppm range.

Conclusions:

  • The novel LC-MS/MS method provides a robust and sensitive approach for quantifying tiamulin residue marker 8-α-hydroxy-mutilin in rabbit tissues.
  • The validated method is suitable for supporting Good Laboratory Practice (GLP) tissue residue depletion studies.
  • This advancement enhances the reliability of analytical data in veterinary drug residue analysis.