Interaction of a novel twin-tailed oxy-diester functionalized surfactant with lysozyme: Spectroscopic and...

Herein, we have examined the interaction of oxy-diester novel twin tailed (gemini) surfactant, 2,2^'-[(oxybis(ethane-1,2-diyl))bis(oxy)]bis(N-hexadecyl-N,Ndimethyl-2-oxoethanaminium) dichloride (C₁₆-E2O-C₁₆) with hen egg white lysozyme (HEWL), utilizing a spectroscopic and molecular docking techniques. Steady-state fluorescence infers ground state C₁₆-E2O-C₁₆-HEWL complex formation. Other spectroscopic results validated the conformational, structural and micro-environmental changes in HEWL upon interaction with C₁₆-E2O-C₁₆. Molecular modeling has shown that C₁₆-E2O-C₁₆ binds in the proximity of hydrophobic moieties (Trp-62/108). We believe the results of the current study will assist in designing the surfactant-enzyme systems for their end use as ingredients in pharmaceutical, cosmetic, drug delivery and industrial compilations. In terms of scientific literature standpoint, it will also enrich and widen the scope of biomacromolecule-surfactant interactions.