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Updated: Feb 18, 2026
CRISPR/Cas12a Multiplex Genome Editing of Saccharomyces cerevisiae and the Creation of Yeast Pixel Art
Published on: May 28, 2019
A standardized toolkit for genetic engineering of CTG clade yeasts
Tatiana A Defosse1, Vincent Courdavault2, Alix T Coste3
1Groupe d'Etude des Interactions Hôte-Pathogène (EA 3142), GEIHP, UNIV. Angers, Université Bretagne-Loire, Angers, France; Université François-Rabelais de Tours, Biomolécules et Biotechnologies Végétales, EA 2106, Tours, France.
Abstract:
We have developed a series of synthetic constructs suitable to genetically manipulate a broad range of yeast species belonging to the fungal CTG clade. This molecular toolbox notably allows heterologous gene expression, single or dual fluorescence labeling and construction of luciferase-expressing strains for bioluminescence imaging.
