Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Enhancing Asthma Management in Emergency Departments: New Approaches Are Vital for Effective Care.

Respirology (Carlton, Vic.)·2026
Same author

Implications of race-neutral equations on interpretation of lung function in Australia.

Internal medicine journal·2026
Same author

Inducible laryngeal obstruction/vocal cord obstruction: still happening under our noses.

Thorax·2026
Same author

Bronchus to Caecum-An Unusual Case of a Migratory Aspirated Dental Implant.

Respirology case reports·2026
Same author

Poor diagnostic performance of flow volume loops for detection of inducible laryngeal obstruction/vocal cord dysfunction.

The journal of allergy and clinical immunology. In practice·2025
Same author

Patient reported outcome measures relevant to asthma remission: scoping review protocol.

MethodsX·2025

Related Experiment Video

Updated: Feb 18, 2026

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy
09:14

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy

Published on: October 19, 2017

10.9K

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy.

Roleen Sharma1, Kim M O'Sullivan2, Stephen R Holdsworth2

  • 1Monash Lung and Sleep, Monash Medical Centre; Monash University.

Journal of Visualized Experiments : Jove
|November 21, 2017
PubMed
Summary
This summary is machine-generated.

Researchers adapted confocal microscopy to visualize macrophage extracellular traps (METs). This method identifies extracellular chromatin and associated proteins, aiding in the study of these crucial immune cell structures in vitro and in vivo.

More Related Videos

In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages
08:08

In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages

Published on: November 1, 2019

10.6K
Neutrophil Extracellular Traps: How to Generate and Visualize Them
14:17

Neutrophil Extracellular Traps: How to Generate and Visualize Them

Published on: February 24, 2010

88.2K

Related Experiment Videos

Last Updated: Feb 18, 2026

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy
09:14

Visualizing Macrophage Extracellular Traps Using Confocal Microscopy

Published on: October 19, 2017

10.9K
In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages
08:08

In Vitro Stimulation and Visualization of Extracellular Trap Release in Differentiated Human Monocyte-derived Macrophages

Published on: November 1, 2019

10.6K
Neutrophil Extracellular Traps: How to Generate and Visualize Them
14:17

Neutrophil Extracellular Traps: How to Generate and Visualize Them

Published on: February 24, 2010

88.2K

Area of Science:

  • Immunology
  • Cell Biology
  • Microscopy

Background:

  • Neutrophil extracellular traps (NETs) are a key immune defense mechanism.
  • Confocal microscopy is the standard for NET visualization.
  • Macrophage extracellular traps (METs) are increasingly recognized but less understood.

Purpose of the Study:

  • To adapt and validate confocal microscopy techniques for visualizing macrophage extracellular traps (METs).
  • To characterize the components and conditions for MET formation.
  • To enable in vitro and in vivo analysis of METs.

Main Methods:

  • Modification of established confocal microscopy protocols.
  • Stimulation of macrophages and comparison with un-stimulated controls.
  • Utilizing image analysis software for quantitative assessment.
  • Inclusion of background and isotype controls for validation.

Main Results:

  • Successful adaptation of confocal microscopy for MET visualization.
  • Identification of extracellular chromatin and associated proteins (granule proteases, citrullinated histones, PAD) in METs.
  • Demonstration of MET presence in both in vitro cell cultures and in vivo lung tissue.

Conclusions:

  • Confocal microscopy is a viable and effective method for defining macrophage extracellular traps (METs).
  • The adapted method allows for detailed characterization of METs and their components.
  • This technique facilitates further research into the role of METs in various biological processes and diseases.