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Patch Clamp

Patch Clamp

Many fundamental cell functions such as muscle contraction and nerve transmission rely on the electrical signals produced by the movement of positively and negatively charged ions across the cell membrane. One competent method to record current flowing across the whole cell or single ion channel is the patch-clamp technique.
In this method, a glass micropipette containing electrolyte solution is tightly sealed against a small portion of the cell membrane. As a result, a patch of the cell...

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Related Experiment Video

Updated: Feb 18, 2026

Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity

Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity

Published on: November 11, 2017

Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity.

Hiroyuki Kida¹, Yuya Sakimoto¹, Dai Mitsushima²

¹Department of Physiology, Yamaguchi University Graduate School of Medicine.

Journal of Visualized Experiments : Jove

|November 21, 2017

Summary

Learning changes neural plasticity in rat brains. Motor learning altered neuron properties, while contextual learning diversified synaptic strength in the hippocampus.

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Last Updated: Feb 18, 2026

Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity

Slice Patch Clamp Technique for Analyzing Learning-Induced Plasticity

Published on: November 11, 2017

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

Electrophysiological and Morphological Characterization of Neuronal Microcircuits in Acute Brain Slices Using Paired Patch-Clamp Recordings

Published on: January 10, 2015

Whole-cell Patch-clamp Recordings in Brain Slices

Whole-cell Patch-clamp Recordings in Brain Slices

Published on: June 15, 2016

Area of Science:

Neuroscience
Cellular Neuroscience
Learning and Memory

Background:

Neural plasticity underlies learning and memory.
The slice patch clamp technique allows detailed investigation of neuronal function.
Understanding how different learning types affect neural circuits is crucial.

Purpose of the Study:

To investigate motor-learning induced plasticity in the primary motor cortex (M1).
To examine contextual-learning induced plasticity in the hippocampus.
To characterize changes in neuronal excitability and synaptic transmission following different learning paradigms.

Main Methods:

Rats were trained on an accelerated rotor rod task for motor learning.
Rats underwent an inhibitory avoidance (IA) task for contextual learning.
Slice patch clamp electrophysiology was used to record from M1 and hippocampal CA1 neurons.

Main Results:

Motor learning induced dynamic changes in M1 pyramidal neuron properties, increasing excitability.
Contextual learning in IA rats led to increased amplitude and diversity of miniature excitatory and inhibitory postsynaptic currents (mEPSCs and mIPSCs) in CA1 neurons.
These synaptic changes were mediated by AMPA and GABAA receptors.

Conclusions:

Motor learning induces significant alterations in neuronal excitability within the primary motor cortex.
Contextual learning promotes postsynaptic diversity at both excitatory and inhibitory synapses in the hippocampus.
The slice patch clamp technique is versatile for studying learning-induced plasticity across different brain regions and learning types.