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Long noncoding RNA LINC00961 inhibits cell invasion and metastasis in human non-small cell lung cancer

Bin Jiang1, Jing Liu2, Yu-Hong Zhang3

  • 1Department of Urology, The Affiliated Jiangyin Hospital of Southeast University Medical College, Wuxi, People's Republic of China.

Biomedicine & Pharmacotherapy = Biomedecine & Pharmacotherapie
|November 22, 2017
PubMed

Insights

The long noncoding RNA LINC00961 is downregulated in non-small cell lung cancer (NSCLC). Lower LINC00961 levels correlate with advanced NSCLC and poor survival, suggesting it acts as a tumor suppressor.

Area of Science:

  • Molecular Biology
  • Oncology
  • Genetics

Background:

  • Long noncoding RNAs (LncRNAs) are increasingly recognized for their roles in cancer, acting as oncogenes or tumor suppressors.
  • Dysregulation of lncRNA expression is a hallmark of various human malignancies, including lung cancer.

Purpose of the Study:

  • To investigate the role of the lncRNA LINC00961 in non-small cell lung cancer (NSCLC).
  • To determine the clinical significance and functional mechanisms of LINC00961 in NSCLC progression.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and Western blot analysis to assess LINC00961 and β-catenin expression.
  • Analysis of the association between LINC00961 expression and clinical parameters in NSCLC patients.
  • In vitro and in vivo experiments to evaluate the effect of LINC00961 on NSCLC cell migration, invasion, and metastasis.
  • Chromatin immunoprecipitation (ChIP) assays to investigate the interaction between LSD1 and the LINC00961 promoter.

Main Results:

  • LINC00961 was significantly downregulated in NSCLC tissues and associated with advanced stage, lymph node metastasis, and shorter survival.
  • LSD1 epigenetically repressed LINC00961 transcription in NSCLC cells.
  • Overexpression of LINC00961 inhibited NSCLC cell migration, invasion, and metastasis.
  • LINC00961 suppressed NSCLC progression partly by affecting β-catenin expression.

Conclusions:

  • Decreased LINC00961 expression is implicated in NSCLC progression.
  • LINC00961 functions as a tumor suppressor in NSCLC.
  • LINC00961 may serve as a potential prognostic biomarker for human NSCLC.
Keywords:
InvasionLINC00961MigrationNSCLCTumor suppressor

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