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CRISPR and crRNAs

CRISPR and crRNAs

Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

The Antiviral System of Bacteria and Archaea: CRISPR

The Antiviral System of Bacteria and Archaea: CRISPR

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeats is a adaptive immune system found in bacteria and archaea that protects against viral infections. This system enables prokaryotic cells to identify, remember, and neutralize foreign genetic elements, primarily bacteriophages, by storing fragments of the invader’s DNA as a genetic memory.The CRISPR immune response begins during an initial infection. Cas (CRISPR-associated) proteins play a central role in this...

CRISPR/Cas9 Genome Editing

CRISPR/Cas9 Genome Editing

The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...

CRISPR

CRISPR

Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...

Defense Against Bacterial Pathogens

Defense Against Bacterial Pathogens

The human immune system is a complex network of cells, tissues, and organs that work together to defend the body against bacterial infections. It consists of various immune cells, each playing a specific role in the defense mechanism.
Phagocytes
Phagocytes are the frontline soldiers of the immune system. They include neutrophils and macrophages. Neutrophils are the most abundant type of white blood cell and are quickly mobilized to the site of infection. Macrophages are larger cells that patrol...

Bacterial Transformation

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In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
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Related Experiment Video

Updated: Feb 18, 2026

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

Published on: August 14, 2021

CRISPR-Based Antibacterials: Transforming Bacterial Defense into Offense.

Adrienne C Greene¹

¹WMD Threats and Aerosol Science Department, Sandia National Laboratories, PO Box 5800 MS 1148, Albuquerque, NM 87185, USA.

Trends in Biotechnology

|November 22, 2017

Summary

Antimicrobial resistance is a global health threat. CRISPR-based antibacterials offer a new, adaptable strategy to combat drug-resistant bacteria and target diverse pathogens.

Keywords:

CRISPR antibacterial antibiotic resistance bacteriophage genome editing nanoparticle

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Last Updated: Feb 18, 2026

Application of CRISPR Interference CRISPRi for Gene Silencing in Pathogenic Species of Leptospira

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Published on: August 14, 2021

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Phage-mediated Delivery of Targeted sRNA Constructs to Knock Down Gene Expression in E. coli

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Published on: March 20, 2016

Area of Science:

Microbiology
Genetics
Biotechnology

Background:

Antimicrobial resistance (AMR) is a significant global health challenge.
Existing treatments are becoming less effective against resistant bacterial strains.

Purpose of the Study:

To explore CRISPR-based antibacterials as a novel therapeutic approach.
To highlight the adaptability of CRISPR technology for combating pathogenic bacteria.

Main Methods:

Utilizing CRISPR-Cas systems for targeted bacterial killing.
Developing adaptable platforms for broad-spectrum antibacterial applications.

Main Results:

CRISPR-based systems demonstrate potential for precise targeting of bacteria.
The adaptable nature of CRISPR allows for rapid development against emerging resistance.

Conclusions:

CRISPR-based antibacterials represent a promising and versatile strategy against AMR.
This technology offers a new avenue for developing effective treatments for bacterial infections.