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P2X7 receptor in spinal tuberculosis: Gene polymorphisms and protein levels in Chinese Han population

Ying Zhou1, Chun-Yan Tan1, Zhi-Jiang Mo2

  • 1Department of Laboratory Medicine, The People's Hospital of Guangxi Autonomous Region, Nanning, China.

Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases
|November 22, 2017
PubMed

Insights

Genetic variations in the P2X7 receptor gene are linked to spinal tuberculosis (TB) susceptibility. Specific P2X7 gene polymorphisms and increased P2X7R expression are associated with higher risks of developing spinal TB.

Area of Science:

  • Genetics
  • Immunology
  • Infectious Diseases

Background:

  • Spinal tuberculosis (TB) is a significant global health concern, representing 1%-5% of all TB cases.
  • Host genetic factors play a crucial role in Mycobacterium tuberculosis (MTB) infection susceptibility.
  • The P2X7 receptor (P2X7R) is implicated in immune responses, cell death, pathogen clearance, and bone remodeling.

Purpose of the Study:

  • To investigate the association between P2X7 gene polymorphisms and protein levels with spinal TB.
  • To determine the role of P2X7R in the pathogenesis of spinal TB.

Main Methods:

  • Genotyping of P2X7 gene -762C>T and 489C>T polymorphisms.
  • Western blot analysis to quantify P2X7R expression in bone and intervertebral disc tissues.
  • Statistical analysis to assess the association between genotypes, allele frequencies, and spinal TB risk.

Main Results:

  • Both -762C>T and 489C>T polymorphisms in the P2X7 gene were significantly associated with spinal TB susceptibility.
  • The -762CC genotype and -762C allele increased the risk of developing spinal TB.
  • The 489T allele (in TT or CT genotypes) was strongly associated with an elevated risk of spinal TB.
  • P2X7R expression was significantly increased in TB-induced bone lesions of spinal TB patients.

Conclusions:

  • P2X7 gene polymorphisms (-762C>T and 489C>T) are risk factors for spinal TB in the studied population.
  • Increased P2X7R expression in bone lesions suggests its involvement in spinal TB pathogenesis.
  • These findings highlight the potential of P2X7R as a biomarker or therapeutic target for spinal TB.
Keywords:
P2X7 receptorPolymorphismSpinal tuberculosisSusceptibility

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