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Updated: Feb 18, 2026

A Simple Protocol for Mapping the Plant Root System Architecture Traits
Published on: February 10, 2023
Differential spatial distribution of miR165/6 determines variability in plant root anatomy
Giovanna Di Ruocco1, Gaia Bertolotti1, Elena Pacifici1
1Department of Biology and Biotechnology, Laboratory of Functional Genomics and Proteomics of Model Systems, Sapienza University of Rome, via dei Sardi, 70-00185 Rome, Italy.
None:
A clear example of interspecific variation is the number of root cortical layers in plants. The genetic mechanisms underlying this variability are poorly understood, partly because of the lack of a convenient model. Here, we demonstrate that Cardamine hirsuta, unlike Arabidopsis thaliana, has two cortical layers that are patterned during late embryogenesis. We show that a miR165/6-dependent distribution of the HOMEODOMAIN LEUCINE ZIPPER III (HD-ZIPIII) transcription factor PHABULOSA (PHB) controls this pattern. Our findings reveal that interspecies variation in miRNA distribution can determine differences in anatomy in plants.
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