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Data on the decreased expression of FOXO1 by miR-1271 in HepG2 hepatocytes

Won-Mo Yang1, Kyung-Ho Min1, Se-Whan Park1

  • 1Department of Biochemistry, Dongguk University College of Medicine, Gyeongju-si, Gyeongsangbuk-do 38067, Republic of Korea.

Data in Brief
|November 22, 2017
PubMed

Insights

MicroRNA-1271 (miR-1271) upregulation in obesity impairs insulin signaling by targeting the FOXO1 gene. This leads to reduced PEPCK transcription, contributing to insulin resistance and type 2 diabetes.

Area of Science:

  • Metabolic diseases
  • Molecular biology
  • Genetics

Background:

  • Obesity and metabolic diseases are linked to insulin resistance.
  • Obesity-induced microRNAs (miRNAs) may contribute to insulin resistance and type 2 diabetes.
  • miR-1271 expression is upregulated in diet-induced obesity.

Purpose of the Study:

  • To investigate the role of miR-1271 in insulin resistance.
  • To identify the direct target of miR-1271.
  • To elucidate the molecular mechanism by which miR-1271 affects insulin signaling.

Main Methods:

  • In silico analysis to predict gene targets.
  • Dual luciferase reporter gene assay to confirm direct binding.
  • Western blot to assess protein expression.
  • Quantitative real-time PCR to measure gene transcription.

Main Results:

  • In silico analysis predicted FOXO1 as a direct target of miR-1271.
  • Dual luciferase assay confirmed miR-1271 binds to the 3'UTR of FOXO1, suppressing its expression.
  • Overexpression of miR-1271 reduced FOXO1 protein levels.
  • Reduced FOXO1 expression led to decreased transcription of PEPCK, a downstream target.

Conclusions:

  • miR-1271 directly targets and suppresses FOXO1 expression.
  • This suppression of FOXO1 by miR-1271 impairs insulin signaling pathways.
  • The findings suggest miR-1271 as a potential therapeutic target for insulin resistance and type 2 diabetes.
Keywords:
FOXO1HepatocyteMicroRNAsmiR-1271

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