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Updated: Feb 18, 2026
Analysis of Fatty Acid Content and Composition in Microalgae
Published on: October 1, 2013
Liquid Chromatography-Mass Spectrometry (LC-MS)-Based Analysis of Molecular Lipids in Algae Samples
Heli Nygren1, Tuulikki Seppänen-Laakso1, Heiko Rischer2
1VTT Technical Research Centre of Finland Ltd., Espoo, Finland.
Abstract:
In this paper, an analytical method for the analysis of molecular lipids in algae samples is reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further characterization of lipids, MS/MS analyses are carried out utilizing either a separate instrument (e.g., LTQ-Orbitrap mass spectrometer) or simultaneous fragmentation with the same instrument. Throughput of the method is over 100 samples/d. The repeatability is good, and the relative standard deviation of spiked samples is <15%.
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