ESI MS for Microsized Bioparticles.
Abdil Ozdemir1, Jung-Lee Lin2, Mustafa Gulfen1
1Department of Chemistry, Faculty of Arts and Sciences, Sakarya University , Esentepe, 54187 Sakarya, Turkey.
Analytical Chemistry
|November 22, 2017
Summary
A new mass spectrometer enables rapid analysis of large bioparticles like cancer cells. This high-throughput system accurately measures cell masses for advanced biological research.
Area of Science:
- Biophysics
- Analytical Chemistry
- Cell Biology
Background:
- Accurate mass measurement of large bioparticles is crucial for biological research.
- Existing mass spectrometry techniques may have limitations in throughput and speed for analyzing biological samples like cells.
Purpose of the Study:
- To develop and validate a high-throughput ESI ion trap mass spectrometer for rapid mass analysis of large bioparticles.
- To demonstrate the instrument's capability in measuring the masses of different sizes of breast cancer cells.
Main Methods:
- Design and implementation of an ESI ion trap mass spectrometer.
- Mass calibration using polystyrene (PS) microparticles (8-15 μm).
- Analysis of MCF-7 breast cancer cells of varying sizes (8-15 μm).
Main Results:
- Successful mass calibration of the instrument using microparticles comparable in size to cancer cells.
- Demonstrated capability to measure the masses of different sizes of MCF-7 breast cancer cells.
- The system is versatile for analyzing various types of particles.
Conclusions:
- The developed ESI ion trap mass spectrometer provides a high-throughput and rapid method for mass analysis of large bioparticles.
- The instrument is suitable for accurate mass determination of biological entities such as cancer cells.
- This technology has broad applicability for particle analysis in diverse scientific fields.
