Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mixture-design optimization of chlorella-chitosan biocomposite films followed by juniper seed extract incorporation for structural and functional reinforcement.

International journal of biological macromolecules·2026
Same author

A Unified Mass Spectrometry Workflow Integrating Bottom-Up and Native Top-Down Proteomics via Nanodroplet Enzymatic Digestion.

Analytical chemistry·2026
Same author

Enhanced label-free SERS detection of uremic toxins in clinical spent dialysate.

Analytica chimica acta·2026
Same author

Solvent-dependent extraction of antioxidants and polyphenolics of quince (Cydonia oblonga Mill.) leaves in conventional and deep eutectic solvents: Response surface methodology, LC-MS phenolic characterization, and mineral assessment.

Food chemistry·2026
Same author

Alterations in trace element profiles in gastric cancer tissues: diagnostic biomarker potential and association with clinical stages.

Environmental geochemistry and health·2026
Same author

Application of ES-DMA-CPC for Comprehensive Molecular Profiling in Hemodialysis: A Focus on β2-Microglobulin.

ACS omega·2025
Same journal

Machine Learning-Assisted Label-Free SERS Decoding of Mitochondrial Molecular Dynamics in Ovarian Granulosa Cells during Aging.

Analytical chemistry·2026
Same journal

Revealing the Regulatory Interplay of NHE1 mRNA and Na<sup>+</sup> in Cancer Cells Using a DNA Nanosensor.

Analytical chemistry·2026
Same journal

Towards Cellular Resolution of Tryptic Peptides in Tissue Sections by MALDI MS Imaging: A Focus on Enzyme Application and Reproducibility.

Analytical chemistry·2026
Same journal

Bioinspired Bilayer Hydrogel Colorimetric Sensor Array for Low-Temperature Food Freshness Analysis.

Analytical chemistry·2026
Same journal

Quartz Crystal Microbalance-Based Point-of-Care Testing Systems: Principles, Device Design, and Applications.

Analytical chemistry·2026
Same journal

Heterojunction Gate-Empowered OPECT Aptasensing: A Valid Protocol for Realizing High Current Gain at Low Electron Donor Dependency.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Feb 18, 2026

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.6K

ESI MS for Microsized Bioparticles.

Abdil Ozdemir1, Jung-Lee Lin2, Mustafa Gulfen1

  • 1Department of Chemistry, Faculty of Arts and Sciences, Sakarya University , Esentepe, 54187 Sakarya, Turkey.

Analytical Chemistry
|November 22, 2017
PubMed
Summary
This summary is machine-generated.

A new mass spectrometer enables rapid analysis of large bioparticles like cancer cells. This high-throughput system accurately measures cell masses for advanced biological research.

More Related Videos

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
10:27

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

9.6K
Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

8.5K

Related Experiment Videos

Last Updated: Feb 18, 2026

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays
09:58

Computer Numerical Control Micromilling of a Microfluidic Acrylic Device with a Staggered Restriction for Magnetic Nanoparticle-Based Immunoassays

Published on: June 23, 2022

2.6K
Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering
10:27

Microfluidic Buffer Exchange for Interference-free Micro/Nanoparticle Cell Engineering

Published on: July 10, 2016

9.6K
Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor
09:49

Fast Enzymatic Processing of Proteins for MS Detection with a Flow-through Microreactor

Published on: April 6, 2016

8.5K

Area of Science:

  • Biophysics
  • Analytical Chemistry
  • Cell Biology

Background:

  • Accurate mass measurement of large bioparticles is crucial for biological research.
  • Existing mass spectrometry techniques may have limitations in throughput and speed for analyzing biological samples like cells.

Purpose of the Study:

  • To develop and validate a high-throughput ESI ion trap mass spectrometer for rapid mass analysis of large bioparticles.
  • To demonstrate the instrument's capability in measuring the masses of different sizes of breast cancer cells.

Main Methods:

  • Design and implementation of an ESI ion trap mass spectrometer.
  • Mass calibration using polystyrene (PS) microparticles (8-15 μm).
  • Analysis of MCF-7 breast cancer cells of varying sizes (8-15 μm).

Main Results:

  • Successful mass calibration of the instrument using microparticles comparable in size to cancer cells.
  • Demonstrated capability to measure the masses of different sizes of MCF-7 breast cancer cells.
  • The system is versatile for analyzing various types of particles.

Conclusions:

  • The developed ESI ion trap mass spectrometer provides a high-throughput and rapid method for mass analysis of large bioparticles.
  • The instrument is suitable for accurate mass determination of biological entities such as cancer cells.
  • This technology has broad applicability for particle analysis in diverse scientific fields.