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Updated: Feb 18, 2026

RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA
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RNA Pull-down Procedure to Identify RNA Targets of a Long Non-coding RNA

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Long noncoding RNA MALAT1 regulates autophagy associated chemoresistance via miR-23b-3p sequestration in gastric

Hu YiRen1, Yu YingCong2,3, You Sunwu1

  • 1Department of General Surgery, The third Clinical Institute Affiliated to Wenzhou Medical University, Wenzhou People's Hospital, Wenzhou, Zhejiang, China.

Molecular Cancer
|November 23, 2017
PubMed
Abstract

Insights

This study reveals that higher levels of MALAT1 in gastric cancer cells promote chemoresistance by increasing autophagy. Silencing MALAT1 sensitizes cancer cells to chemotherapy, offering new therapeutic strategies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Chemoresistance is a significant challenge in cancer treatment.
  • Understanding chemoresistance mechanisms is crucial for developing effective therapies.

Purpose of the Study:

  • To investigate the role of MALAT1 in chemoresistance in gastric cancer.
  • To elucidate the mechanism by which MALAT1 influences autophagy and chemoresistance.

Main Methods:

  • Quantitative real-time PCR (qPCR) to measure lncRNA expression.
  • Transfection of MALAT1 shRNAs and overexpression vectors in gastric cancer cells.
  • In vitro and in vivo assays to assess the functional role of MALAT1.

Main Results:

  • Chemoresistant gastric cancer cells exhibit elevated MALAT1 levels and increased autophagy.
  • MALAT1 knockdown reduces chemo-induced autophagy and sensitizes cells to chemotherapy.
  • MALAT1 functions as a competing endogenous RNA for miR-23b-3p, impacting ATG12 expression and promoting autophagy.

Conclusions:

  • A novel mechanism of long noncoding RNA (lncRNA)-regulated autophagy-related chemoresistance in gastric cancer was identified.
  • MALAT1 plays a key role in mediating chemoresistance through autophagy modulation.
  • This finding provides new insights into overcoming chemoresistance in gastric cancer.
Keywords:
AutophagyChemoresistanceGastric cancerMALAT1lncRNA

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