Non-labeled lensless micro-endoscopic approach for cellular imaging through highly scattering media

Area of Science:

• Biomedical Optics
• Medical Imaging Technology
• Endoscopy

Background:

• Conventional endoscopic imaging is limited by scattering media and resolution.
• Existing techniques like Optical Coherence Tomography (OCT) have limitations in long-term imaging and mechanical constraints.

Purpose of the Study:

• To present a lensless, minimally invasive endoscopic imaging approach with enhanced capabilities.
• To demonstrate imaging beyond highly scattering media with improved resolution and signal-to-noise ratios.

Main Methods:

• A miniature, lensless endoscope employing a scanning optical setup.
• Post-processing techniques including fast overlapping scans and super-resolution algorithms.
• Utilizing overlapping scans to enhance non-scattered signal to scattered noise ratio.

Main Results:

• Achieved single-cell imaging resolution.
• Demonstrated successful imaging through highly scattering media (e.g., mimicking blood capillaries and skin).
• Obtained enhanced signal-to-noise levels and resolution compared to conventional methods.

Conclusions:

• The developed imaging approach overcomes limitations of traditional endoscopes and imaging techniques.
• This method enables high-quality imaging through scattering biological tissues.
• Potential applications for enhanced *in vivo* biomedical diagnostics and minimally invasive procedures.