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Related Concept Videos

CRISPR01:59

CRISPR

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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CRISPR/Cas9 Genome Editing01:28

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The CRISPR-Cas system serves as a bacterial defense mechanism against invading genetic elements such as viruses and plasmids, forming the foundation for its adaptation as a powerful genome-editing tool. Originally discovered in prokaryotes, this system has been repurposed to revolutionize genetic engineering across a wide range of organisms, including plants, animals, and humans. The core component, Cas9, is an endonuclease derived from Streptococcus pyogenes, capable of introducing...
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CRISPR and crRNAs02:53

CRISPR and crRNAs

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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
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Related Experiment Video

Updated: Feb 18, 2026

A Rapid and Facile Pipeline for Generating Genomic Point Mutants in C. elegans Using CRISPR/Cas9 Ribonucleoproteins
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DNA nanomapping using CRISPR-Cas9 as a programmable nanoparticle.

Andrey Mikheikin1, Anita Olsen1, Kevin Leslie1

  • 1Department of Physics, Virginia Commonwealth University, Richmond, 23284, VA, USA.

Nature Communications
|November 23, 2017
PubMed
Summary
This summary is machine-generated.

Researchers developed a novel DNA nanomapping technique using CRISPR-Cas9 nanoparticles and high-speed atomic force microscopy (HS-AFM). This method precisely maps genetic translocations, complementing whole-genome sequencing for disease diagnostics.

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Area of Science:

  • Genomics
  • Molecular Biology
  • Nanotechnology

Background:

  • Whole-genome sequencing with short reads has limitations in high-resolution physical mapping.
  • Existing methods struggle to fill technical gaps left by sequencing technologies.

Purpose of the Study:

  • To introduce two technical advances in DNA nanotechnology and single-molecule genomics.
  • To develop a novel high-speed AFM-based physical mapping technique for DNA.
  • To demonstrate its application in detecting specific genetic translocations.

Main Methods:

  • Developed a DNA labeling technique using CRISPR-Cas9 nanoparticles.
  • Utilized DVD optics for high-speed AFM imaging of DNA molecules.
  • Applied the "nanomapping" method to lymph node biopsies from follicular lymphoma patients.

Main Results:

  • Successfully demonstrated high-speed AFM-based physical mapping of DNA.
  • Precisely detected and mapped BCL2-IGH translocations in follicular lymphoma patient samples.
  • Showcased the feasibility of the "nanomapping" technique for clinical diagnostics.

Conclusions:

  • The novel HS-AFM "nanomapping" technique offers high-resolution DNA physical mapping.
  • This method is complementary to existing sequencing and physical mapping approaches.
  • It holds potential for precise detection of genetic alterations in diseases like follicular lymphoma.