Brush-border TEA transport in intact proximal tubules and isolated membrane vesicles

The efflux of the organic cation, tetraethylammonium (TEA), across proximal cell luminal membranes was studied using intact, perfused rabbit proximal tubules and isolated rabbit cortical brush-border membrane vesicles (BBMV). Increases of either the extravesicular H+ concentration (from pH 7.5 to pH 6.5) or the extravesicular concentration of unlabeled TEA (from 0.1 to 0.5 mM) increased the rate of efflux of radioactively labeled TEA from BBMV. Similarly, when proximal tubules were preloaded with labeled TEA and then submerged in a mineral oil bath, a rapid increase in either the H+ concentration (from pH 7.5 to pH 5.8) or the TEA concentration (from 0 to 1 mM) of the tubular perfusate produced an acute increase in efflux of the labeled TEA across the luminal brush-border membrane. These results with intact tubules and isolated membranes are consistent with previous suggestions that TEA transport across the brush border of proximal tubule cells involves a carrier-mediated countertransport process and represent the first demonstration with intact proximal tubules that the secretory flux of TEA occurs by an exchange with H+.