Related Experiment Video
Updated: Feb 18, 2026
10:23
Standardization of a Cytometric Bead Assay Based on Egg-Yolk Antibodies
Published on: May 19, 2023
1.3K
Method for quantitative detection of FAM19A4 by flow cytometry using latex beads as solid carrier
Ting Li1, Wenyan Wang2, Yingying Cheng3
1Department of Immunology, School of Basic Medical Sciences, Peking University Health Science Center, Key Laboratory of Medical Immunology, Ministry of Health (Peking University), Beijing, 100191, China; Peking University Center for Human Disease Genomics, Beijing, 100191, China.
Journal of Bioscience and Bioengineering
|November 24, 2017
Summary
A new sandwich immunoassay detects FAM19A4 (family with sequence similarity 19 member A4), a cytokine involved in immune responses. This method accurately quantifies FAM19A4 in biological fluids, aiding further research.
Area of Science:
- Immunology
- Biochemistry
- Cell Biology
Background:
- Family with sequence similarity 19 member A4 (FAM19A4), also known as TAFA4, is a secretory protein primarily found in the central nervous system.
- FAM19A4 expression is significantly increased in monocytes and macrophages stimulated by lipopolysaccharide (LPS).
- FAM19A4 acts as a novel cytokine ligand for formyl peptide receptor 1 (FPR1), influencing macrophage functions.
Purpose of the Study:
- To develop a sensitive and specific immunoassay for the quantitative detection of FAM19A4 in biological samples.
- To characterize the performance of the developed assay in terms of sensitivity, specificity, precision, and recovery.
- To validate the assay's ability to detect native FAM19A4 secreted by stimulated immune cells.
Main Methods:
- Development of a sandwich immunoassay utilizing latex beads as a solid carrier for flow cytometry detection.
- Assay principle based on enzyme-linked immunosorbent assay (ELISA).
- Quantification of FAM19A4 in various biological matrices, including cell culture supernatants.
Main Results:
- The developed immunoassay demonstrated good performance across a wide detection range (39-10,000 pg/mL).
- The assay exhibited excellent specificity, good precision, and favorable recovery rates in different matrices.
- The method successfully detected native FAM19A4 secreted by THP-1 cells stimulated with phorbol 12-myristate 13-acetate (PMA) and LPS.
Conclusions:
- A novel and robust sandwich immunoassay for FAM19A4 detection has been successfully developed.
- This assay provides a valuable tool for the quantitative analysis of FAM19A4 in biological fluids.
- The method will significantly facilitate future research on the role of FAM19A4 in biological processes and disease.
