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Updated: Feb 18, 2026
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A Nonsequencing Approach for the Rapid Detection of RNA Editing
Published on: April 21, 2022
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Epitranscriptomic profiling across cell types reveals associations between APOBEC1-mediated RNA editing, gene
Violeta Rayon-Estrada1,2, Dewi Harjanto1, Claire E Hamilton1,2,3
1Laboratory of Lymphocyte Biology, The Rockefeller University, New York, NY 10065.
Summary
Apolipoprotein B mRNA-editing enzyme (APOBEC1) modifies transcripts in monocytes, impacting gene expression and cellular functions. This RNA editing is crucial for maintaining immune cell homeostasis.
Area of Science:
- Molecular Biology
- Genetics
- Immunology
Background:
- Epitranscriptomics involves dynamic, reproducible mRNA modifications affecting gene expression.
- The functional significance of epitranscriptomic modifications is not fully understood.
- RNA editing is a key epitranscriptomic process with incompletely defined roles.
Purpose of the Study:
- To investigate the role of Apolipoprotein B mRNA-editing enzyme, catalytic polypeptide-1 (APOBEC1) in RNA editing.
- To determine how APOBEC1-mediated editing impacts gene expression, cellular processes, and immune cell function.
- To explore the relevance of APOBEC1 in monocyte development and homeostasis.
Main Methods:
- Analysis of APOBEC1 and RBM47 in various tissues.
- Focus on RNA editing events in monocyte 3'UTRs.
- Assessment of editing impact on translation, RNA stability, and localization.
- Enrichment analysis of editing targets in cellular processes like phagocytosis.
- Survey of APOBEC1 expression in bone marrow progenitors.
Main Results:
- APOBEC1 and RBM47 mediate robust RNA editing across tissues.
- Most editing events occur in the 3'UTR, modulating translation without affecting RNA stability or localization.
- Cellular processes including phagocytosis and transendothelial migration involve editing targets.
- Bone marrow progenitors express APOBEC1, and its loss impacts progenitor cells.
- APOBEC1-mediated editing diversifies the transcriptome, fine-tuning protein expression in monocytes.
Conclusions:
- APOBEC1-mediated RNA editing is essential for fine-tuning protein expression in monocytes.
- Transcriptome diversification through editing plays a role in maintaining innate immune cell homeostasis.
- APOBEC1 is critical for the function of monocyte progenitor cells and mature monocytes.
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