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Updated: Feb 18, 2026

Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria

Models of Murine Vaginal Colonization by Anaerobically Grown Bacteria

Published on: May 25, 2022

Infant Rabbit Colonization Competition Assays.

Yang Fu¹, John J Mekalanos¹

¹Department of Microbiology and Immunobiology, Harvard Medical School, Boston, USA.

|November 25, 2017

Summary

This summary is machine-generated.

A new infant rabbit model accurately mimics human cholera, aiding the study of enteric pathogens like Vibrio cholerae. This assay is crucial for developing new vaccines and therapeutics against bacterial intestinal colonization.

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Last Updated: Feb 18, 2026

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Depletion of Specific Cell Populations by Complement Depletion

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Published on: February 5, 2010

Area of Science:

Microbiology
Infectious Diseases
Pathogenesis Research

Background:

Enteric pathogens, including Vibrio cholerae and enteropathogenic Escherichia coli, cause severe diarrheal diseases.
Understanding bacterial intestinal colonization mechanisms is vital for developing effective treatments and vaccines.
Existing models do not fully replicate human disease symptoms or facilitate high-throughput screening.

Purpose of the Study:

To introduce a modified Infant Rabbit Colonization Competition Assay for studying enteric bacterial pathogenesis.
To provide a more clinically relevant animal model for Vibrio cholerae infections compared to traditional models.
To enable high-throughput screening for bacterial colonization factors.

Main Methods:

A modified nonsurgical animal model using a competition assay in 2-day-old New Zealand white rabbits.
Inoculation of mixed wild-type and mutant bacterial strains to measure competitive index.
Assessment of colonization capacity relative to wild-type strains in the gastrointestinal tract.

Main Results:

The infant rabbit model exhibits clinical and histologic signs closely resembling human cholera.
The assay allows for the measurement of bacterial colonization capacity through competitive indices.
The model's capacity for larger bacterial inputs supports high-throughput screening methods like Tn-Seq.

Conclusions:

The Infant Rabbit Colonization Competition Assay is a valuable tool for studying enteric bacterial pathogenesis.
This model offers improved clinical relevance for Vibrio cholerae research.
The assay facilitates the discovery of bacterial colonization factors and aids in therapeutic and vaccine development.