Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture01:26

Sputum Studies I: Gram Stain, cytology, and Acid-fast smear and culture

725
Sputum studies are a critical part of diagnosing and treating numerous respiratory conditions. These studies involve obtaining sputum samples for analysis to identify pathogenic organisms and assess the presence of abnormal cells indicative of malignant conditions. This lesson will delve into three fundamental sputum studies: Gram Stain, Cytology, and Acid-fast Smear and Culture.
Gram Stain
The Gram Stain is an integral part of sputum studies. It involves the staining of sputum, which permits...
725
Special Staining Techniques01:13

Special Staining Techniques

1.4K
Specialized staining techniques play a vital role in microbiology by enabling the visualization of specific bacterial structures that remain undetectable with standard microscopy methods. These techniques not only enhance the structural visualization of bacterial cells but also provide critical insights into their pathogenicity and classification. Additionally, they support diagnostic and research endeavors in microbiology by identifying key bacterial features.Capsule Staining for Virulence...
1.4K
Simple Staining Technique01:24

Simple Staining Technique

4.3K
OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...
4.3K
Fixation and Sectioning01:03

Fixation and Sectioning

8.1K
Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...
8.1K
Differential Staining Technique01:26

Differential Staining Technique

2.5K
Differential staining is an essential microbiological technique that exploits variations in cell wall structures to classify and identify microorganisms. It facilitates the distinction of bacteria, aiding in diagnostic and research applications. Two of the most widely used differential staining methods are Gram staining and acid-fast staining, both of which rely on the chemical and structural differences in bacterial cell walls.Gram Staining TechniqueGram staining differentiates bacteria by...
2.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antifibrotic monocyte activation by nanoparticles resolves murine pulmonary fibrosis.

Biomaterials·2026
Same author

Gut microbiota associate with disease severity and survival in idiopathic pulmonary fibrosis.

American journal of respiratory and critical care medicine·2026
Same author

Viral-specific induction of cellular and soluble urokinase plasminogen activator receptor (suPAR) expression.

Journal of immunology (Baltimore, Md. : 1950)·2026
Same author

Molecular endotypes predict differential response to immunosuppressant therapy in non-idiopathic pulmonary fibrosis interstitial lung disease.

American journal of respiratory and critical care medicine·2026
Same author

"Immunomodulatory nanoparticles elicit antifibrotic monocyte activation to resolve murine pulmonary fibrosis".

bioRxiv : the preprint server for biology·2026
Same author

Targeted lung <i>Lactobacillus johnsonii</i> intervention alleviates virus-induced fibrosis post-HCT through PD-L1/PD-1 signaling.

Science advances·2026
Same journal

Editorial: Technologies for RNA Detection.

Bio-protocol·2026
Same journal

One-Step Affinity Purification of MarathonRT Reverse Transcriptase for RNA Sequencing Applications.

Bio-protocol·2026
Same journal

Enhanced RNA-Seq Expression Profiling and Functional Enrichment in Non-model Organisms Using Custom Annotations.

Bio-protocol·2026
Same journal

Using Combined Fluorescent In Situ Hybridization With Immunohistochemistry to Co-localize mRNA in Diverse Neuronal Cell Types.

Bio-protocol·2026
Same journal

Stepwise Protocol for Alternative Splicing Analysis in Single-Cell SMART-Seq2 RNA-Seq Data.

Bio-protocol·2026
Same journal

Enriching Bacteria-Specific RNA From Host Samples Before NGS With Transcript-Capture.

Bio-protocol·2026
See all related articles

Related Experiment Video

Updated: Feb 18, 2026

A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation
08:46

A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation

Published on: July 26, 2017

14.0K

Lung Section Staining and Microscopy.

Xiaofeng Zhou1, Bethany B Moore1

  • 1Department of Internal Medicine, Pulmonary and Critical Care Medicine Division, University of Michigan, Ann Arbor, Michigan, USA.

Bio-Protocol
|November 25, 2017
PubMed
Summary
This summary is machine-generated.

This study details a protocol for three common staining techniques: immunofluorescent staining, hematoxylin and eosin staining, and Masson

Keywords:
AntibodiesFrozen tissue sectionsHematoxylin and eosin stainingImmunofluorescent stainingMasson’s trichrome stainingParaffin-embedded tissue sections

More Related Videos

Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices
12:04

Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

Published on: May 9, 2018

14.8K
Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections
07:17

Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections

Published on: October 6, 2023

7.8K

Related Experiment Videos

Last Updated: Feb 18, 2026

A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation
08:46

A Standardized Method for Measuring Internal Lung Surface Area via Mouse Pneumonectomy and Prosthesis Implantation

Published on: July 26, 2017

14.0K
Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices
12:04

Assessment of the Cytotoxic and Immunomodulatory Effects of Substances in Human Precision-cut Lung Slices

Published on: May 9, 2018

14.8K
Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections
07:17

Author Spotlight: Studying Spatial Protein Expression Using Agarose Embedded Lung Tissue Sections

Published on: October 6, 2023

7.8K

Area of Science:

  • Histopathology
  • Immunohistochemistry
  • Cellular Biology

Background:

  • Accurate tissue staining is crucial for diagnosing lung diseases.
  • Standardized protocols ensure reproducible results in histological analysis.

Purpose of the Study:

  • To present a comprehensive protocol for key lung tissue staining methods.
  • To facilitate consistent application of immunofluorescent staining, hematoxylin and eosin staining, and Masson's trichrome staining.

Main Methods:

  • Detailed procedure for immunofluorescent staining on lung sections.
  • Step-by-step guide for hematoxylin and eosin staining.
  • Methodology for Masson's trichrome staining of lung tissue.

Main Results:

  • The protocol provides clear instructions for each staining technique.
  • Successfully stained lung sections were obtained using the described methods.

Conclusions:

  • This protocol enables reliable application of multiple histological and immunohistochemical staining techniques on lung sections.
  • It serves as a valuable resource for researchers and diagnosticians working with lung tissue samples.